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BioTechnologia
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1/2016
vol. 97
 
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abstract:
RESEARCH PAPERS

Interactive effects of plant growth regulators and explants on direct shoot regeneration of Viola odorata

Arash Mokhtari
1
,
Mohsen Zarei
1
,
Behnaz Samsamzadeh
2
,
Kosar Moradi
1

  1. Department of Plant Tissue Culture, Branch of Central Region of Iran, Agricultural Biotechnology Research Institute of Iran (ABRII), Isfahan, Najafabad, Iran
  2. Department of Agricultural Biotechnology, Payam Noor University of Isfahan, Isfahan, Iran
BioTechnologia vol. 97(1) C pp. 33-39 C 2016
DOI: https://doi.org/10.5114/bta.2016.58542
Online publish date: 2016/04/20
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The aim of the study was to develop a protocol for in vitro shoot regeneration based on a solid Murashige and Skoog (MS) basal medium supplemented with various concentrations of plant growth regulators (PGRs) like Indole-3-butyric acid (IBA), kinetin (KIN) or benzylaminopurine (BAP) using leaf, petiole, root, and crown disk segments of a valuable medicinal plant Viola odorata. The best percentage of direct shoot regeneration (67.50%) and the maximum number of micro-shoots (9.50) were obtained with crown explants on MS medium containing IBA (0.5 mgA l!1) combined with BAP (3 or 4 mg A l!1). The best elongation (5.25 cm) of micro-shoots was observed in crown explants on a medium containing 1 mg A l!1 gibberellic acid (GA3) followed by GA3 at 0.5 mg A l!1 and similar explants. Irrespective of the concentrations of GA3, the minimum length of shoots was observed in leaf explants. A half-strength MS medium containing activated charcoal and supplemented with 1.5 mg A l!1 IBA resulted in the best rooting (78%) of elongated shoots. All acclimatized plantlets survived and showed a normal growth. The study reports a simple and applicable protocol for micropropagation of V. odorata through direct organogenesis.
keywords:

crown explants, direct regeneration, GA3, Viola odorata


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