eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
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3/2021
vol. 46
 
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abstract:
Experimental immunology

LncRNA ZEB1-AS1 knockdown alleviates oxidative low-density lipoprotein-induced endothelial cell injury via the miR-590-5p/ HDAC9 axis

Jinpeng Zhong
1
,
Bin Cheng
2
,
Li Yang
3
,
Guanlan Li
2
,
Yunzhong Yuan
2
,
Gang Luo
2
,
Zhiping Shu
2
,
Hong Jiang
1

  1. Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, Hubei, China
  2. Department of Cardiology, The People’s Hospital of China Three Gorges University, Yichang, Hubei, China
  3. Department of Neurology, The People’s Hospital of China Three Gorges University, Yichang, Hubei, China
Cent Eur J Immunol 2021; 46 (3): 325-335
DOI: https://doi.org/10.5114/ceji.2021.108767
Online publish date: 2021/10/19
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Oxidative low-density lipoprotein (ox-LDL) is thought to induce vascular endothelial cell injury, which contributes to the aetiopathogenesis of atherosclerosis (AS). Several previous reports have identified that lncRNA ZEB1-AS1 participates in the regulatory mechanisms of endothelial cell injury, but the potential interaction mechanism between ZEB1-AS1 and miR-590-5p in ox-LDL-induced endothelial cell damage is not clear. ZEB1-AS1 and miR-590-5p expression were tested by quantitative real-time polymerase chain reaction (qRT-PCR) in ox-LDL-treated endothelial cells. The proliferation and apoptosis were determined by MTT and Annexin V/PI double-staining assay, respectively. The protein expression of HDAC9, tumor necrosis factor  (TNF-), cleaved caspase-3, and cleaved PARP were measured by western blot analysis. Dual-luciferase reporter and RIP assays affirmed the functional targets of ZEB1-AS1. ZEB1-AS1 expression was upregulated in ox-LDL-treated HUVECs, and miR-590-5p was lessened in a dose- or time-depended manner, respectively. Knockdown of ZEB1-AS1 facilitated ox-LDL-treated endothelial cell proliferation and inhibited cell apoptosis. Moreover, miR-590-5p was directly targeted via ZEB1-AS1 in ox-LDL-treated HUVECs. ZEB1-AS1 silencing attenuated ox-LDL-induced cell injury via regulation of miR-590-5p expression. Furthermore, HDAC9 reversed the influence of miR-590-5p on propagation and apoptosis of ox-LDL-induced endothelial cells. Knockdown of ZEB1-AS1 alleviates ox-LDL-induced endothelial cell injury by regulating the miR-590-5p/HDAC9 axis.
keywords:

lncRNA ZEB1-AS1, miR-590-5p, HDAC9, ox-LDL, atherosclerosis
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