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ISSN: 0860-7796
BioTechnologia
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3/2019
vol. 100
 
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abstract:
RESEARCH PAPERS

Lofty frequency and reproducible plant regeneration from mature nodal explants of ‘‘Egusi’’melon (Citrullus colocynthis L.)

Prabhu Thangavel
1
,
Sisubalan Natarajan
2, 3
,
Velmurugam Shanmugam
1
,
Sheik Mohamed Sulaiman
1
,
Ravikumar Ramasamy
1

  1. P.G. and Research Department of Botany, Jamal Mohamed College (Autonomous), Khaja Nagar, Tamil Nadu, India
  2. P.G. and Research Department of Botany, J.J. College of Arts and Science (Autonomous), Pudukkottai, Tamil Nadu, India
  3. Key Laboratory of Molecular Epigenetics of MOE and Institute of Genetics and Cytology, Northeast Normal University, Changchun, China
BioTechnologia vol. 100 (3) C pp. 263–272 C 2019
DOI: https://doi.org/10.5114/bta.2019.87585
Online publish date: 2019/09/26
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The present study attempts to explain a mass culture protocol for large scale multiple shoot bud regeneration from nodal explants of Citrullus colocynthis L. The highest shoot bud induction (94.9%) without attaining callus was observed in Murashige and Skoog’s (MS) medium supplemented with benzyl amino purine (BA)in which the combination of benzyl amino purine BAP (1.0 mg/l), kinetin (0.5 mg/l), gibberellic acid (1.5 mg/l) produced more shoots/explants. This study strongly suggests that the pre-eminence of BA and other cytokinins in combination with GA3 was found to be essential for a swift multiple shoot bud induction as well as an enhanced rate of shoot proliferation in C. colocynthis. A full-strength MS medium, supplemented with indole 3 butyric acid IBA (1.5 mg/l) combined with BA (1.5 mg/l), had the maximum rooting response and the regenerated plantlets were successfully established in the field with an 80% survival rate.
keywords:

nodal explants, surface sterilization, mercuric chloride, shoot bud, BA, multiple shoot, IBA, GA3, rooting


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