eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
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3/2017
vol. 98
 
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abstract:
RESEARCH PAPERS

Lunasin – a bioactive peptide from triticale (X Triticosecale Wittmack) seeds, inhibits proliferation of cancer HeLa and SK-OV-3 cells

Mariola E. Galbas
1
,
Filip Porzucek
1
,
Marek Selwet
2
,
Agnieszka Nowak
1
,
Ryszard Slomski
1

  1. Department of Biochemistry and Biotechnology, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences, Poznań, Poland
  2. Department of General and Environmental Microbiology, Faculty of Agronomy and Bioengineering, Poznań University of Life Sciences, Poznań, Poland
BioTechnologia vol. 98(3) C pp. 219-224 C 2017
DOI: https://doi.org/10.5114/bta.2017.70800
Online publish date: 2018/01/02
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The presented study demonstrates the influence of lunasin, a bioactive peptide isolated from seeds of winter triticale, on the proliferation of neoplastic cells. In order to conduct investigations, 2S fraction low-molecular-weight albumin proteins were extracted. SDS-PAGE electrophoresis separation of extracts indicated the presence of a 5 kDa peptide in the crude extract, which corresponded to the weight of lunasin. The next stage of protein purification involved ion-exchange column chromatography in an FPLC system on DEAE Sepharose Fast Flow. The separation of biomolecules by means of 2D electrophoresis confirmed that a peptide with an isoelectric point was present in the area, with a pH of about 5.5 and molecular mass of about 5 kDa. The presence of lunasin in the purified samples was confirmed by Western blot. The lunasin peptide affected the proliferation of cervical cancer (HeLa) and ovarian cancer (SK-OV-3) cell lines. The average decrease in the HeLa cell proliferation rate was 17%. The SK-OV-3 cell line was more susceptible to inhibition by the protein fraction containing lunasin than HeLa cells, with an average decrease in the proliferation rate of 48%.
keywords:

triticale, lunasin, Western blotting, HeLa, SK-OV-3 cell lines


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