eISSN: 2449-8238
ISSN: 2392-1099
Clinical and Experimental Hepatology
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3/2020
vol. 6
 
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abstract:
Original paper

MicroRNA-499 rs3746444 polymorphism in Egyptian children with biliary atresia

Eman Gawish
1
,
Elhamy Abd El-Monem
1
,
Mona El-Abd
1
,
Gihan Ahmed Sobhy
2
,
Heba Ghanem
1

  1. Department of Clinical Pathology, National Liver Institute – Menoufia University, Menoufia, Egypt
  2. Department of Pediatric Hepatology, Gastroenterology and Nutrition, National Liver Institute – Menoufia University, Menoufia, Egypt
Clin Exp HEPATOL 2020; 6, 3: 263–269
DOI: https://doi.org/10.5114/ceh.2020.99526
Online publish date: 2020/09/30
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Introduction
We aimed to evaluate the association of microRNA-499 rs3746444 polymorphism and biliary atresia (BA) risk and its correlation with clinic-pathologic features of BA.

Material and methods
This study was performed on 300 Egyptian children (100 BA cases, 100 cases with cholestatic liver diseases other than BA and 100 healthy controls). Routine laboratory investigations, clinical examination and abdominal ultrasound were done. All infants were genotyped for miR-499 single nucleotide polymorphisms (SNPs) (rs3746444 A>G) by real-time polymerase chain reaction (PCR) fluorescence detection on a Rotor Gene Real Time PCR System (QIAGEN, GmbH) using fluorescent labeled probes.

Results
The AG genotype was the most prevalent genotype of miR-499 rs3746444 among the studied groups. A significantly higher frequency of the rs3746444 G allele was found in the BA cases than the other groups (odds ratio = 1.62). This polymorphism was also correlated with the degree of fibrosis in BA cases (p < 0.05). The miR-499 rs3746444 polymorphism (GG genotype) was significantly associated with severe form of BA and bad prognosis after the Kasai operation (p < 0.05). miR-499 rs3746444 polymorphism had no effect on the clinic-pathological features or the liver function status in the non-BA group.

Conclusions
There is an association between the miR-499 SNP genotypes and the occurrence of BA. The variant allele G is the predominant allele in the BA group and is associated with severe liver inflammation and bad prognosis after the Kasai operation.

keywords:

biliary atresia, cholestatic liver diseases, Kasai operation, miR-499
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