Molecular cloning and sequencing analysis of the interferon β from <i>Coturnix</i>

Bei Zheng; Wei-Shan Chang

doi:10.5114/ceji.2014.42118

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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1/2014
vol. 39

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abstract:

Molecular cloning and sequencing analysis of the interferon β from Coturnix

Bei Zheng

,

Wei-Shan Chang

(Centr Eur J Immunol 2014; 39 (1): 25-29

DOI: https://doi.org/10.5114/ceji.2014.42118

Online publish date: 2014/04/17

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One pair of primers was designed according to Gallus and Meleagris gallopavo interferon β (IFN-β) sequences published in GenBank. The primers and RNA extraction from the spleen of Coturnix were used to amplify Coturnix IFN-β cDNA by real-time polymerase chain reaction (RT-PCR). The product was cloned into pEasy-T1 vector. Evaluating recombinant plasmid by PCR and restriction enzyme digestion. Sequence the cloning sequences, comparing the sequencing results by NCBI. We successfully got a Coturnix IFN-β partial sequence. The sequence was subtyped and put to homologous analysis. The results suggested the homology of IFN-β gene of Coturnix and chicken (88.7%), the homology of IFN-β gene of Coturnix and Anas platyrhynchos (72.5%), the homology of IFN-β sequence registered in GenBank. The analysis of the genetic tree showed that the relationship of Coturnix and chicken IFN-β had a high homology. It can be seen that in this study we successfully got a partial sequence of IFN-β of quail.

keywords:

Molecular cloning and sequencing analysis of the interferon β from Coturnix

Bei Zheng , Wei-Shan Chang

Coturnix, IFN-β, gene cloning, gene sequence analysis

Bei Zheng

,

Wei-Shan Chang