eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
Newsletter
Enter your email:
 
Search
Editorial Policies
Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

Without publication fees

without publication fees

Open access

without publication fees

Journal integrated with

integrated with publons
1/2017
vol. 98
 
Share:
Send email
Share:
abstract:
RESEARCH PAPERS

Molecular identity and cytotoxicity of Lenzites quercina macrofungus extracts toward cancer cell lines

Olusola Clement Ogidi
1
,
Victor Olusegun Oyetayo
1
,
Bamidele Juliet Akinyele
1
,
Omonike Oluyemisi Ogbole
2
,
Johnson Adekunle Adeniji
3
,
Bolaji Bosede Oluremi
4

  1. Department of Microbiology, Federal University of Technology, Akure, Nigeria
  2. Department of Pharmacognosy, Faculty of Pharmacy, University of Ibadan, Ibadan, Nigeria
  3. W.H.O. Polio Laboratory, Department of Virology, College of Medicine, University of Ibadan, Ibadan, Nigeria
  4. Department of Pharmaceutical Microbiology, University of Ibadan, Ibadan, Nigeria
BioTechnologia vol. 98(1) C pp. 25-32 C 2017
DOI: https://doi.org/10.5114/bta.2017.66614
Online publish date: 2017/05/25
View full text Get citation
 
PlumX metrics:
The medicinal uses of wild macrofungi have been attributed to their accumulated bioactive compounds. Several mushrooms have been reported to possess antitumor activity, but little, in this regard, is known about Lenzites quercina collected from Akure in Nigeria. Hence, the molecular identification and cytotoxic activity of extracts obtained from raw and fermented Lenzites quercina were assessed. The macrofungus Lenzites quercina was identified using Internal Transcribed Spacers (ITS) sequence analysis. The basic local alignment search tool (BLAST) analyzed on NCBI GenBank data revealed that the Lenzites species from Nigeria – accession number, JF689829.1 – was closely related to Lenzites quercina (a 100% relationship match). The cytotoxic activity of raw and fermented Lenzites quercina extracts was tested against human cervical cancer (HeLa), habdomyosarcoma (RD) and African green monkey kidney (VERO) cell lines. A tetrazolium yellow 3-(4,5-dimethyl thiazol-2-yl)-2,5- diphenyl tetrazolium bromide (MTT) colorimetric assay was used to evaluate the reduction in viability of cell cultures with or without the extracts of Lenzites quercina. Extracts of Lenzites quercina exhibited cytotoxic activity (6.0-84.5%) against the tested cancer cell lines (HeLa, VERO and RD). The concentration of the bioactive compounds in the crude extract ranged from 0.01 to 1000 μg/ml. The results revealed that bioactive compounds in Lenzites quercina possess cytotoxic properties. These bioactive compounds may be isolated and used as alternative therapies to currently available anticancer drugs.
keywords:

Lenzites quercina, chemotherapy, cancer cells, MTT


Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here
Hosted by Termedia sp. z o.o.