Multicolor flow cytometry immunophenotyping
and characterization of aneuploidy in pediatric
B-cell precursor acute lymphoblastic leukemia

Magdalena Pierzyna-Świtała; Łukasz Sędek; Jan Kulis; Bogdan Mazur; Katarzyna Muszyńska-Rosłan; Andrzej Kołtan; Mariola Woszczyk; Maciej Niedźwiecki; Agnieszka Mizia-Malarz; Grażyna Karolczyk; Monika Lejman; Joanna Trelińska; Wanda Badowska; Katarzyna Derwich; Tomasz Ociepa; Iwona Malinowska; Bernarda Kazanowska; Jerzy Kowalczyk; Tomasz Szczepański

doi:10.5114/ceji.2021.109794

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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abstract:

Clinical immunology

Multicolor flow cytometry immunophenotyping and characterization of aneuploidy in pediatric B-cell precursor acute lymphoblastic leukemia

Magdalena Pierzyna-Świtała

¹

,

Łukasz Sędek

¹

,

Jan Kulis

²

,

Bogdan Mazur

¹

,

Katarzyna Muszyńska-Rosłan

³

,

Andrzej Kołtan

⁴

,

Mariola Woszczyk

⁵

,

Maciej Niedźwiecki

⁶

,

Agnieszka Mizia-Malarz

⁷

,

Grażyna Karolczyk

⁸

,

Monika Lejman

⁹

,

Joanna Trelińska

¹⁰

,

Wanda Badowska

¹¹

,

Katarzyna Derwich

¹²

,

Tomasz Ociepa

¹³

,

Iwona Malinowska

¹⁴

,

Bernarda Kazanowska

¹⁵

,

Jerzy Kowalczyk

¹⁶

,

Tomasz Szczepański

²

Department of Microbiology and Immunology, Medical University of Silesia, Zabrze, Poland
Department of Pediatric Hematology and Oncology, Medical University of Silesia, Katowice, Poland
Department of Pediatric Oncology, Medical University of Bialystok, Białystok, Poland
Department of Pediatric Hematology and Oncology, Collegium Medicum in Bydgoszcz, Mikolaj Kopernik University in Toruń, Bydgoszcz, Poland
Center of Pediatrics and Oncology, Chorzow, Poland
Department of Pediatrics, Hematology, Oncology and Endocrinology, Medical University of Gdańsk, Gdańsk, Poland
Department of Pediatrics, Medical University of Silesia, Katowice, Poland
Regional Specialistic Pediatric Hospital, Kielce, Poland
Department of Pediatric Hematology, Oncology and Transplantology, University Children’s Hospital, Genetic Diagnostic Laboratory, Lublin, Poland
Department of Pediatrics, Oncology and Hematology, Medical University of Lodz, Lodz, Poland
Department of Pediatric Hematology and Oncology, Children’s Hospital in Olsztyn, Olsztyn, Poland
Department of Pediatric Hematology, Oncology, Transplantology, Poznan University of Medical Science, Poznań, Poland
Department of Pediatrics, Hematology and Oncology, Pomeranian Medical University, Szczecin, Poland
Department of Pediatric Hematology and Oncology, Medical University of Warsaw, Warsaw, Poland
Department of Transplantology, Pediatric Oncology and Hematology, Wroclaw Medical University, Wrocław, Poland
Department of Pediatric Hematology and Oncology, Medical University of Lublin, Lublin, Poland

Cent Eur J Immunol 2021; 46 (3): 365-374

DOI: https://doi.org/10.5114/ceji.2021.109794

Online publish date: 2021/10/19

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AMA

Pierzyna-Świtała M, Sędek Ł, Kulis J, et al. Multicolor flow cytometry immunophenotyping
and characterization of aneuploidy in pediatric
B-cell precursor acute lymphoblastic leukemia. Central European Journal of Immunology. 2021;46(3):365-374. doi:10.5114/ceji.2021.109794.

APA

Pierzyna-Świtała, M., Sędek, Ł., Kulis, J., Mazur, B., Muszyńska-Rosłan, K.,  & Kołtan, A. et al. (2021). Multicolor flow cytometry immunophenotyping
and characterization of aneuploidy in pediatric
B-cell precursor acute lymphoblastic leukemia. Central European Journal of Immunology, 46(3), 365-374. https://doi.org/10.5114/ceji.2021.109794

Chicago

Pierzyna-Świtała, Magdalena, Łukasz Sędek, Jan Kulis, Bogdan Mazur, Katarzyna Muszyńska-Rosłan, Andrzej Kołtan,  and Mariola Woszczyk et al. 2021. "Multicolor flow cytometry immunophenotyping
and characterization of aneuploidy in pediatric
B-cell precursor acute lymphoblastic leukemia". Central European Journal of Immunology 46 (3): 365-374. doi:10.5114/ceji.2021.109794.

Harvard

Pierzyna-Świtała, M., Sędek, Ł., Kulis, J., Mazur, B., Muszyńska-Rosłan, K., Kołtan, A., Woszczyk, M., Niedźwiecki, M., Mizia-Malarz, A., Karolczyk, G., Lejman, M., Trelińska, J., Badowska, W., Derwich, K., Ociepa, T., Malinowska, I., Kazanowska, B., Kowalczyk, J.,  and Szczepański, T. (2021). Multicolor flow cytometry immunophenotyping
and characterization of aneuploidy in pediatric
B-cell precursor acute lymphoblastic leukemia. Central European Journal of Immunology, 46(3), pp.365-374. https://doi.org/10.5114/ceji.2021.109794

MLA

Pierzyna-Świtała, Magdalena et al. "Multicolor flow cytometry immunophenotyping
and characterization of aneuploidy in pediatric
B-cell precursor acute lymphoblastic leukemia." Central European Journal of Immunology, vol. 46, no. 3, 2021, pp. 365-374. doi:10.5114/ceji.2021.109794.

Vancouver

Pierzyna-Świtała M, Sędek Ł, Kulis J, Mazur B, Muszyńska-Rosłan K, Kołtan A et al. Multicolor flow cytometry immunophenotyping
and characterization of aneuploidy in pediatric
B-cell precursor acute lymphoblastic leukemia. Central European Journal of Immunology. 2021;46(3):365-374. doi:10.5114/ceji.2021.109794.

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The aim of this study was to assess the incidence of DNA aneuploidy in Polish children with B-cell precursor acute lymphoblastic leukemia (BCP-ALL) and the relationship between aneuploidy and immunological phenotype, age, leukocyte count, S-phase fraction (SPF) and early response to induction chemotherapy assessed by the percentage of residual blast cells in bone marrow aspirates. The study group consisted of 267 patients. DNA content and immunophenotype were assessed in the bone marrow before treatment using multicolor flow cytometry (FC). DNA aneuploidy was detected in 50/267 (19%) patients. High hyperdiploidy was found to be associated with lower leukocyte count (p = 0.006) and common ALL immunophenotype. Flow cytometry analysis revealed that high hyperdiploid BCP-ALL patients showed significantly higher expression of CD9, CD20, CD22, CD58, CD66c, CD86 and CD123 antigens as compared to other groups of ploidy. In contrast, CD45 showed decreased expression. The percentage of leukemic blasts at diagnosis was lower in high hyperdiploid BCP-ALL cases than in diploid (79% vs. 85.7%, p = 0.001). The difference in minimal residual disease (MRD) levels on day 15 and 33 of induction therapy between analyzed groups was not significant.

This study showed that high hyperdiploidy is associated with lower WBC count and specific immunological phenotype. Flow cytometric evaluation of expression of selected antigens can be used for fast identification of markers of aneuploidy in pediatric BCP-ALL, before genetic tests results are available. Understanding the biological significance of aneuploidy in leukemia can potentially be exploited therapeutically using targeted therapies against specific blast cell subclones.

keywords:

Multicolor flow cytometry immunophenotyping and characterization of aneuploidy in pediatric B-cell precursor acute lymphoblastic leukemia

flow cytometry, aneuploidy, acute leukemia, DNA index, hyperdiploidy