eISSN: 1897-4309
ISSN: 1428-2526
Contemporary Oncology/Współczesna Onkologia
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4/2024
vol. 28
 
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abstract:
Original paper

NBAT1/miR-21 axis in progression of colorectal cancer and impact of PVT-1 polymorphism on miR-145 expression level and its clinical significance

Ghada Ayeldeen
1
,
Ahmed K. Zaki
2
,
Eman Amer
3
,
Zeinab Abdellatif
4
,
Olfat G. Shaker
1
,
Mohamed Said
5
,
Amr M. Abdelhamid
5

  1. Department of Medical Biochemistry and Molecular Biology, Faculty of Medicine, Cairo University, Cairo, Egypt
  2. Department of Clinical and Chemical Pathology, National Cancer Institute, Cairo University, Cairo, Egypt
  3. Department of Biochemistry, Faculty of Pharmacy, Ahram Canadian University, Egypt
  4. Department of Endemic Medicine, Faculty of Medicine, Cairo University, Cairo, Egypt
  5. Department of Biochemistry, Faculty of Pharmacy, October University for Modern Sciences and Arts (MSA), 6th of October, Egypt
Contemp Oncol (Pozn) 2024; 28 (4): 304–317
DOI: https://doi.org/10.5114/wo.2024.146961
Online publish date: 2025/01/15
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Introduction:
Patients with colorectal cancer (CRC) have a higher chance of survival when the disease is detected and treated effectively at an early stage. Plasmacytoma variant translocation 1 (PVT-1), an oncogenic lncRNA, and neuroblastoma associated trans­cript 1 (NBAT1), a tumor suppressor lncRNA, have been linked to CRC progression, acting as competing endo­genous RNAs to the tumor suppressor miRNA-145 and oncomiRNA-21. The aim of the current study was to construct a competing endogenous RNA (ceRNA) associated with CRC. In addition, we aimed to investigate the impact of single nucleotide polymorphisms in the rs13255292 lncRNA PVT-1 on miR-145 expression levels and the lncRNA-NBAT1/miR-21 axis in the progression of CRC.

Material and methods:
Bioinforma­tic analysis was performed to determine differentially expressed genes (DEGs), differentially expressed micro­RNAs (DEMs), and differentially expressed lncRNAs (DELs) in CRC. PVT-1 rs13255292 C/T was genotyped and serum PVT-1, NBAT-1, miRNA-145 and miRNA-21 were assessed by qPCR in 85 CRC patients, 80 AP, and 85 controls.

Results:
The frequencies of the PVT-1 rs13255292 CT/TT genotype and T al­le­- le were significantly elevated in the CRC group compared to the controls. PVT-1 serum levels significantly increased due to the presence of the T allele in the studied groups, which was associated with downregulation of the miR-145 tumor suppressor. Also, the expression of NBAT-1 was significantly down-expressed, while that of oncomiR-21 was significantly elevated.

Conclusions:
Bioinformatics analyses provides effective identification of potential lncRNAs linked with CRC. PVT-1/miR-145 and NBAT1/miR-21 are being investigated as potential non-invasive diagnostic biomarkers for CRC.

keywords:

colorectal cancer, PVT-1, NBAT-1, miRNA-145, miRNA-21
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