No differences in miRNA expression in the stroma  of ERG+ and ERG– prostate cancers

Gracjan Wątor; Maria Kołton-Wróż; Paweł Wołkow; Łukasz Bełch; Piotr Chłosta; Joanna Szpor; Agnieszka Klimkowska; Katarzyna Nejman; Alicja Józkowicz; Aleksandra Piechota-Polańczyk; Krzysztof Okoń

doi:10.5114/pjp.2023.134304

eISSN: 2084-9869
ISSN: 1233-9687

Polish Journal of Pathology

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4/2023
vol. 74

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abstract:

Original paper

No differences in miRNA expression in the stroma of ERG+ and ERG– prostate cancers

Gracjan Wątor

¹

,

Maria Kołton-Wróż

¹

,

Paweł Wołkow

¹

,

Łukasz Bełch

²

,

Piotr Chłosta

²

,

Joanna Szpor

^{3, 4}

,

Agnieszka Klimkowska

⁴

,

Katarzyna Nejman

^{3, 4}

,

Alicja Józkowicz

⁵

,

Aleksandra Piechota-Polańczyk

⁵

,

Krzysztof Okoń

^{3, 4}

Center for Medical Genomics OMICRON, Jagiellonian University Medical College, Kraków, Poland
Department of Urology, Jagiellonian University Medical College, Kraków, Poland
Department of Pathomorphology, Jagiellonian University Medical College, Kraków, Poland
Department of Pathomorphology, University Hospital in Kraków, Kraków, Poland
Department of Medical Biotechnology, Faculty of Biochemistry, Biophysics and Biotechnology, Jagiellonian University, Kraków, Poland

Pol J Pathol 2023; 74 (4): 265-270

DOI: https://doi.org/10.5114/pjp.2023.134304

Online publish date: 2024/01/17

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Prostate cancer (PC) is one of the most common cancers in males. A significant proportion of PCs bear TMPRSS2-ETS translocation and overexpress ERG transcription factor, allowing classification into ERG+ and ERG– groups, which differ in several features including the tumor microenvironment. The aim of the study was to verify whether they differ in expression of the miRNA in the microenvironment. The material consisted of 150 radical prostatectomies. Immunohistochemistry (IHC) for ERG was done using a routine method. FISH for TMPRSS2-ETS translocation was done with a ZytoLight SPEC ERG/TMPRSS2 TriCheck Probe. From each case, a representative section was selected, and tumor and non-tumor were microdissected with the LMD7000 device. RNA was isolated using the RNeasy Mini Kit system (Qiagen) and miRNA libraries were prepared with the NEBNext Multiplex Small RNA Library Prep Set for Illumina and their sequencing was performed on the NexSeq 500. Statistical analysis was done with Statistica and R software. When analyzing the expression of miRNAs some differences could be seen, but after correction for multiple comparisons was applied, these were found to be non- significant.

keywords:

No differences in miRNA expression in the stroma of ERG+ and ERG– prostate cancers

Gracjan Wątor 1 , Maria Kołton-Wróż 1 , Paweł Wołkow 1 , Łukasz Bełch 2 , Piotr Chłosta 2 , Joanna Szpor 3, 4 , Agnieszka Klimkowska 4 , Katarzyna Nejman 3, 4 , Alicja Józkowicz 5 , Aleksandra Piechota-Polańczyk 5 , Krzysztof Okoń 3, 4

prostate cancer, TMPRSS2-ETS translocation, miRNA

Gracjan Wątor

¹

,

Maria Kołton-Wróż

¹

,

Paweł Wołkow

¹

,

Łukasz Bełch

²

,

Piotr Chłosta

²

,

Joanna Szpor

^{3, 4}

,

Agnieszka Klimkowska

⁴

,

Katarzyna Nejman

^{3, 4}

,

Alicja Józkowicz

⁵

,

Aleksandra Piechota-Polańczyk

⁵

,

Krzysztof Okoń

^{3, 4}