eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
Newsletter
Enter your email:
 
Search
Editorial Policies
Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

Without publication fees

without publication fees

Open access

without publication fees

Journal integrated with

integrated with publons
4/2016
vol. 97
 
Share:
Send email
Share:
abstract:
RESEARCH PAPERS

Obtaining doubled haploid lines of the Lr19 gene using anther cultures of winter wheat genotypes

Dorota Weigt
1
,
Angelika Kiel
1
,
Jerzy Nawracała
1
,
Agnieszka Tomkowiak
1
,
Danuta Kurasiak-Popowska
1
,
Idzi Siatkowski
2
,
Bogusława Ługowska
3

  1. Department of Genetics and Plant Breeding, Poznań University of Life Sciences, Poznań, Poland
  2. Department of Mathematical and Statistical Methods, Poznań University of Life Sciences, Poznań, Poland
  3. DANKO Plant Breeding Ltd.
BioTechnologia vol. 97(4) C pp. 285-293 C 2016
DOI: https://doi.org/10.5114/bta.2016.64545
Online publish date: 2017/01/13
View full text Get citation
 
PlumX metrics:
Leaf rust pathogens are able to overcome plants’ resistance by the appearance of new races. Therefore, an introduction of leaf rust resistance genes into highyielding wheat genotypes is essential. One of such genes is Lr19 (leaf rust resistance gene) that could be a source of resistance in polish breeding programs. The aim of this study was to obtain doubled haploid lines of the Lr19 gene in anther cultures of winter wheat genotypes. In the experiment, F3 generation plants from two cross-combinations were used: genotype T39 × Ozon and genotype T36 × Hondia (T36 and T39 lines carry Lr19 gene). Three combinations of growth regulators such as 2,4-D; 2,4-D and dicamba; and 2,4-D and kinetin were applied. A total of 13,550 anthers were tested. The embryo-like structures were formed at an average frequency of 9.95% and were mainly influenced by the genotype, not by the composition of hormones in induction media. Regeneration frequency of green plants was recorded to be 1.45%. The

highest frequency of green plant regeneration was observed on induction media supplemented with 2,4-D and dicamba reaching 1.79%. Spontaneous doubled haploids tested by laser flow cytometry were detected in 33.95% of plants. The frequency of the formation of embryo-like structure was mainly influenced by the genotype, not by the composition of hormones in induction media.
keywords:

wheat, leave rust, androgenesis, regeneration frequency, doubled haploids


Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here
Hosted by Termedia sp. z o.o.