Ocena obrazowa grubości mięśnia prostego brzucha wśród dorosłych: określenie potencjalnych zależności antropometrycznych

Introduction

Skeletal muscle architecture is the primary determinant of muscle function [1, 2]. The knowledge of muscle architecture is of great practical importance in understanding the relationship between muscle structure, force, and extension ability [3]. Muscle architecture has been mainly characterised by fascicle length, pennation angle, and the thickness of the muscle [4–6].
Muscle thickness can be described as the cross-sectional diameter of the belly that extends from aponeurosis to aponeurosis. Its characterisation as part of the muscle architecture cannot be overemphasised, because of its role in the comparative assessment of gross morphology of muscles [7]. Muscle thickness is very important clinically in the assessment of atrophy and hypertrophy in neuromuscular diseases [8], in the estimation of general muscle volume [9], in the quantification of amyotrophic lateral sclerosis [10], and in the categorisation of the intensity of relationship with activity levels in cerebral palsy [11]. In the trunk, muscle thickness is used in the monitoring of trunk strengthening exercises necessary for therapeutic alleviation of low back pain [12–15] and other musculoskeletal disorders of the trunk.
The rectus abdominis (RA), as a flap-like muscle, is an important postural muscle that plays a role in movement and in stabilisation of the trunk. It helps the lower ribs, especially during expiration. It compresses the abdomen, supports the viscera (intestine), and is very important in weight lifting and other sports. The muscle, as a myocutaneous flap, has been used in numerous surgical reconstructions: in the reconstruction of scrotum [16]; reconstruction of the breast [17, 18]; closure of sternal defects [19]; in head and neck reconstructive surgery [20]; in the treatment of enterocutaneous fistula [21]; in the correction of pharyngoesophageal defects [22]; in soft tissue reconstruction of the hand [23]; in treatment of complex urethrovaginal fistulae [24, 25]; in the reconstruction of full-thickness thoracic wall defects [26], and in the reconstruction of ankle and foot defects [27]. It is therefore obvious that detailed assessment of the thickness of the rectus abdominis muscleis very important, given its background in these myocutaneous reconstructive procedures.
Previous studies have tried to assess the thickness of the rectus abdominis muscle (RAMT) in different populations. Ikezoe et al. [28] reported that RAMT varies with the age and inactivity of anindividual. This report on the effect of age was also documented earlier by other findings [29–32]. In a different assessment, Balius et al. [33] reported that sportsmen had thicker muscles than non-sportsmen, emphasising the effect of the degree of activity/inactivity on muscle thickness. Also, other factors like posture, nutrition, sex, and pregnancy have been reported to affect the thickness of the rectus abdominis muscle [31, 32, 34, 35].
Anthropometric relationships of some abdominal muscles have been assessed by some studies. Rostami et al. [36] studied the relationship between the lateral abdominal muscles (the transversus abdominis, internal oblique, and external oblique muscles) and body mass index (BMI), abdominal skin fold thickness, and waist circumference and opined that these relationships were important in the assessment of fatness. Other studies, even though they were based on muscles of the extremity, reported a relationship between muscle thickness and some anthropometric variables and established that these myoanthropometric relationships were important in sex categorisation, anthropological categorisation, and lean body mass [7, 37].
Despite these reports, no study has comprehensively established any sort of myoanthropometric relationship using the rectus abdominis in our Sub-Saharan African environment, and since the muscle flap forms a reliable material in numerous reconstructive surgeries, it is absolutely necessary to assess the RAMT in an apparently healthy adult population with a view to creating and documenting information that will serve as a normogram for the African population, as well as a robust platform for comparison in clinical settings and a guidepost to studies on the biomechanical inclinations of the muscle. The RAMT assessment will be done using ultrasound (US). Most assessments of muscles are done with the use of ultrasound because real-time sonography enables in vivo muscle scanning and offers an assurance for a realistic determination of changes in muscle architecture [6, 38].

Material and methods

Study design

This study made use of B-mode ultrasound in measuring the rectus abdominis muscle thickness and was carried out within the Abakaliki metropolis, Ebonyi State, Southeast Nigeria.

Study centre

The Lifescan Ultrasound Centre located at Felix Memorial hospital, No. 6 Hilltop Road, Abakaliki, Ebonyi state, Southeast Nigeria was used. Patients from within the Abakaliki metropolis, Ogoja Metropolis, and beyond are received in this ultrasound and imaging centre. It is headed by a highly experienced radiographer.

Participants

A total of 120 healthy subjects made up of 60 males and 60 females, who gave their written consent, were recruited for this study after are search information sheet regarding the aims of the study, imaging procedure, rights of the subjects, and safety of ultrasound was given to each of them. They were selected from a pool of students at Ebonyi State University (EBSU), who hail from Ebonyi State, Southeast Nigeria (Ebonyians) and were between the ages of 19 and 30 years.

Criteria for selection of subjects

The age range of the participants was from 19 to 30 years. The subjects were all students of Ebonyi State University (EBSU), who are Ebonyians without any history of low back pain in the preceeding three months, no history of performing core stability exercises in the past three months [36], including all forms of rigorous sports, and no potential metabolic diseases. This is to avoid any possible effect of these ailments on the musculoskeletal system. Also, none of the subjects had ever been pregnant or was pregnant at the time of the study, because pregnancy seems to affect the thickness of the rectus abdominis muscle [35]. Prior to the conduction of the main study, a pilot study was undertaken involving 6 subjects (12 RAM) to identify any potential errors in the planned study layout.

Instrumentation

An ultrasound system with a 7.5-MHZ linear trans-ducer (Siemens sonoline SL-1, 1998 model) was used to measure the RAMT.

Scanning protocol

The subjects comprised 120 subjects, 60 males and 60 females. Each subject lay in a supine position while the thickness of the RA was measured at 3 cm lateral to the umbilicus (Figure 1). A 7.5-MHz ultrasound transducer was used to assess the RAMT on both sides. The RAMT was measured accurately using on-screen callipers, taking the distance between the inside edges of the fascia.
To avoid inter-observer variability, the same investigator took all the measurements. During the examination, care was taken to maintain the same standardised position of the subjects and the exact location of the transducer, which was held perpendicular to the skin surface using the minimum pressure required to achieve a clear image. To improve acoustic coupling, a water-soluble transmission gel was placed over the scan head.
The activity of the abdominal muscle is modulated by respiration, i.e. the thickness of the rectus abdominis increases during expiration [31, 39, 40]. Therefore, readings were taken at consistent points at the end of a released expiration (when the respiratory muscles were relaxed).

Anthropometric measurements

Materials for the anthropometric measurements include a calibrated weighing balance that was used to measure the weights of the subjects while the subject was standing erect with minimal clothing; a wall-mounted stadiometer with movable headpiece was used for the measurement of heights of subjects; and a plastic measuring tape (60 inch/150 cm, made in Shanghai, China) was used to assess abdominal circumference at the level of the umbilicus with the subject being in a relaxed state.

Body mass index Body mass index (BMI) was calculated using the formula BMI = weight (kg)/(height (m))2.

Conicity index This was calculated using the formula designed by Teylor et al. [41]: waist circumference/ Conicity index = (0.109 × square root of weight/height).

Body surface area This was calculated by the formula below Body surface area (BSA) = 0.007184 × W0.426 × H0.721 [42].

Statistical analysis

The statistical package for the social sciences (SPSS) software version 16.0 was used to analyse the generated data. Data was expressed as mean ± standard deviation. Student’s t-test was used to compare means, and Pearson’s correlation was used to express relationships.

Ethical approval

In line with the Helsinki Declaration of 1989, ethical approval was obtained from the Research/Ethics committee of the Faculty of Basic Medical Sciences, Ebonyi State University, Abakaliki, Southeast Nigeria.

Results

Table 1 above shows that values of age, height, weight, BMI, BSA, WC, and CI are greater in males than in females, except for the conicity index, which is greater in females than in males (1134.68 in females vs. 1125.66 in males). The line graphs in Figures 1 Aand 1 B show that the rectus abdominis thickness is greater in males than in females. Its thickness is also greater in the right side in males than it is in females.
The mean rectus abdominis thickness in young adult Nigerians is 14.52 ±2.72 mm. The thickness of the RAM in both sexes shows that the right sides are significantly higher than the left sides (p < 0.05). Even in the general population, RAT was still thicker in males than in females (p < 0.05), as can be seen in the Table 2. These values corroborate the outcome of Figures 2 and 3.
Table 3 shows the difference between right rectus abdominis thickness (RRAT) and left rectus abdominis thickness (LRAT) in the general population, in males and in females. The difference in thickness between the right and left sides was not significant in the general population (p = 0.132). However, in males the RRAT was significantly greater than the LRAT (p < 0.05). This significant level of difference was not seen in females (p = 0.272), who presented thicker LRAT than RRAT (Table 4).
Pearson’s correlation coefficient test showed that there was significant correlation between the RAT and age, weight, height, BMI, BSA, and CI (p < 0.5), as shown in the Table 5.

Discussion

Trunk musculoskeletal disorders, especially low back pain, have become common among different occupational groups. Trunk muscle biomechanics have been employed in the management of some of these disorders. The rectus abdominis muscle cannot be left out since it contributes greatly in stabilising the trunk.
The thickness of the Rectus abdominis muscle was assessed in a young adult population, and the results showed a mean thickness of 14.52 ±2.72 mm for the entire population and 15.24 ±2.58 mm and 13.19 ±2.51 mm for male and female subjects, respectively. The RAMT values obtained in this study are higher than the results from previous studies. Rankin et al. [30] reported a mean thickness of 10.2 ±1.60 mm for the right RAM using ultrasound. Kim et al. [43] reported RAT at the level of the Xiphoid processas 9.58 ±2.11 mm and 9.73 ±2.06 mm for the right and left sides, respectively. They also reported a thickness of 10.26 ±1.83 mm and 10.26 ±1.85 mm for right and left sides, respectively, at the level of the umbilicus in an adult female population. These observed disparities might be associated with environmental, racial, and ethnic factors. Another important factor may be due to the degree of physical activity of our subjects, having used relatively active adults at the peak of muscle development.
Generally, these values are of clinical importance in reconstructive surgeries involving the muscle. Phillips et al. [44] proposed that a proper thickness of the muscle when used in myocutaneous flap reconstructive surgeries is important for safe elevation and gives an insight into the functionality of the major supplying arterial pedicles. Consequently, reduced muscle thickness may be an indication of the setting in of muscle wasting following possible damage to the supplying pedicles with an associated reduction in its functional role in the trunk. On the other hand, a thin RAM will naturally leave the pedicles unprotected and prone to damage during extraction of myocutaneous flaps. Thus, information on the thickness of the rectus abdominis muscle is clinically very important and can be helpful to the surgeon [45].
Other results from this study reveal that the RAMT in males was significantly thicker than those of females (Table 3; p < 0.05). This is another manifestation of sexual dimorphism, which in this case is a consequence of male masculinity as a result of hormonal attributes and, possibly, a higher degree of physical activity. In line with our findings, Kanehisa et al. [29] earlier reported the effect of sex on the thickness of the abdominal muscles, which may indicate the degree of force generation and sexual dimorphism.
The results also showed that there was no significant difference between the RRAT and LRAT in the sample population and in female subjects. However, the RRAT was greater than the LRAT in the male subjects (Table 5). This significant difference in males could be a result of their postural tilt, which initiates a greater thickness inclined to the right side, following prolonged phases of high degrees of physical activity. It could be called masculinity-induced laterality of the muscle. The non-significant difference in the female subjects may be due to a possible lack of forceful activity associated with femininity.
Pearson’s correlation coefficient showed a high degree of connectivity between the RAT and the assessed anthropometric variables: age, height, weight, BMI, BSA, WC, and CI (Tables 4 and 5). The correlation with age is in line with the findings of Kanehisa et al. [29], and this clearly infers the effect of age on the thickness of RAM. Its relationship with weight could be explained in its trunk stabilising role associated with the weight of the trunk. Its extent in the anterior abdominal wall obviously contributed to its significant relationship with the height of the subjects, and because of these relationships with weight and height, it is significantly associated with the BMI and BSA. Its relationship with WC and CI may indicate its position in the anthropometric assessment of the trunk, bearing in mind the connection between WC, CI, and intra-abdominal adipose tissue thickness, subcutaneous adipose tissue thickness as reported by Njoku [46], and in the evaluation of fatness as reported by Rostami et al. [36].
The myoanthropometric relationships observed in this study are the first set of relationships of the rectus abdominis muscle reported for a Sub-Saharan African population. Hence, its novelty in this environment will initiate investigations of the clinical and biomechanical aspects of the muscle with regards to its role in lifting sports among Africans. Since the value of RAT in this study is seen to be higher than previously reported values, it is necessary to explore possible biomechanical and sports-related advantages that could be associated with these results in our environment. The reported myoanthropometric relationships could also be used in the assessment of trunk mass and the muscle’s possible role in the mechanical aspects of respiration.

Conclusions

Males have a significantly higher RAMT than females. The values of RAMT seen in this study are higher than those of previous studies. The RAMT correlates positively with age, weight, height, BMI, BSA, WC, and CI. These myoanthropometric relationships will serve as a guidepost in the clinical and biomechanical assessment of the muscle and its role in the assessment of trunk mass and stability in an African population.

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Address for correspondence:

Ogugua Augustine Egwu
Federal University, Ndufu-Alike Ikwo
P.M.B 1010, 234 Abakaliki, Nigeria
Phone: +2348034518414
E-mail: egwuoc@gmail.com