eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
Newsletter
Enter your email:
 
Search
Editorial Policies
Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

Without publication fees

without publication fees

Open access

without publication fees

Journal integrated with

integrated with publons
1/2023
vol. 104
 
Share:
Send email
Share:
abstract:
RESEARCH PAPERS

Optimization of mycelial growth and cultivation of wild Ganoderma sinense

Luyen Thi Nguyen
1
,
Ve Van Le
1
,
Bich Thuy Thi Nguyen
1
,
Huyen Trang Thi Nguyen
1
,
Anh Dong Tran
1
,
Nghien Xuan Ngo
1

  1. Faculty of Biotechnology, Vietnam National University of Agriculture, Hanoi, Vietnam
BioTechnologia vol. 104(1) ∙ pp. 65–74 ∙ 2023
DOI: https://doi.org/10.5114/bta.2023.125087
Online publish date: 2023/03/27
View full text Get citation
 
PlumX metrics:
Ganoderma sinense, a well-known medicinal macrofungus of Basidiomycetes, is widely used in traditional medicine for promoting health and longevity in East Asia. The fruiting bodies of G. sinense contain polysaccharides, ergosterol, and coumarin, which have antitumor, antioxidant, and anticytopenia activities. Mushroom cultivation requires suitable conditions for the formation of fruiting bodies and yield. However, little is known about the optimal culture conditions for mycelial growth and cultivation of G. sinense. In this study, the successful cultivation of a G. sinense strain collected from the wild was reported. The optimal culture conditions were identified by examining one factor at a time. The results of this study revealed that the nutritional requirements for the optimal mycelial growth of G. sinense were fructose (15 g/l) as the carbon source and yeast extract (1 g/l) as the nitrogen source. The optimal pH and temperature for G. sinense were 7 and 25–30̊C, respectively. The mycelia grew fastest in treatment II (69% rice grains + 30% sawdust + 1% calcium carbonate). G. sinense produced fruiting bodies under all tested conditions and showed the highest biological efficiency (2.95%) in treatment B (96% sawdust, 1% wheat bran, 1% lime). In summary, under optimal culture conditions, G. sinense strain GA21 showed satisfactory yield and a high potential for commercial cultivation.
keywords:

Ganoderma sinense, mycelial growth, spawn, lingzhi mushroom


Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here
Hosted by Termedia sp. z o.o.