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ISSN: 0860-7796
BioTechnologia
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4/2019
vol. 100
 
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abstract:
RESEARCH PAPERS

Optimizing the biosynthesis of renewable polyhydroxyalkanoate copolymer containing 3-hydroxyvalerate by Massilia haematophila using statistical modeling

Jong T. Kiun
1
,
Tan S. M. Amelia
1
,
Kai Hee Huong
2
,
Al-Ashraf Abdullah Amirul
2, 3
,
Kesaven Bhubalan
1, 3

  1. Faculty of Science and Marine Environment, Universiti Malaysia Terengganu, Kuala Nerus, Terengganu, Malaysia
  2. School of Biological Sciences, Universiti Sains Malaysia, Penang, Malaysia
  3. Malaysian Institute of Pharmaceuticals and Nutraceuticals (Ipharm), NIBM, Penang, Malaysia
BioTechnologia vol. 100 (4) C pp. 359–371 C 2019
DOI: https://doi.org/10.5114/bta.2019.90237
Online publish date: 2019/12/25
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Polyhydroxyalkanoate (PHA) is a microbial storage polymer that is naturally produced by certain bacteria. This is the first study on the ability of this particular species Massilia haematophila to synthesize a PHA copolymer containing 3-hydroxyvalerate (3HV) monomer. Using the statistical design on Massilia haematophila UMTKB-2, this study highlights the optimization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV), copolymer production for shaken-flask cultivation. Moreover, the mechanical and thermal features of the polymers were determined. The production of P(3HB-co-3HV) by Massilia haematophila UMTKB-2 using optimal conditions provided by response surface methodology (RSM) yielded 5.0 g/l of P(3HB-co-7 mol% 3HV), which was higher than the value obtained from unoptimized conditions such as 4.40 g/l of P(3HB-co-4mol% 3HV). This result showed a 14% increase in copolymer concentration and a two-fold increase in 3HV composition. In this study, the P(3HB-co-3HV) synthesized was determined as a block copolymer and its thermal properties were better than P(3HB). Using RSM, the optimization conditions were successfully obtained for this bacterium, and this result is a starting platform for additional studies of a larger scaled PHA production from Massilia haematophila UMTKB-2 using bioreactors.
keywords:

3-hydroxyvalerate, biorenewable, Massilia haematophila, optimization, polyhydroxyalkanoate, response surface methodology


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