Overexpression of cathepsin K and vascular endothelial growth factor in chronic venous ulcerations

Paweł Kolano; Igor Bednarski; Aleksandra Lesiak; Małgorzata Skibińska; Olga Stasikowska-Kanicka; Marian Danilewicz; Joanna Narbutt

doi:10.5114/ada.2020.94840

eISSN: 2299-0046
ISSN: 1642-395X

Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii

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abstract:

Original paper

Overexpression of cathepsin K and vascular endothelial growth factor in chronic venous ulcerations

Paweł Kolano

¹

,

Igor A. Bednarski

²

,

Aleksandra Lesiak

²

,

Małgorzata Skibińska

²

,

Olga Stasikowska-Kanicka

³

,

Marian Danilewicz

³

,

Joanna Narbutt

²

Department of General and Oncological Surgery, Tomaszow Health Centre, Tomaszow Mazowiecki, Poland
Department of Dermatology, Paediatric Dermatology and Oncology, Medical University of Lodz, Lodz, Poland
Department of Pathology, Medical University of Lodz, Lodz, Poland

Adv Dermatol Allergol 2020; XXXVII (2): 234–239

DOI: https://doi.org/10.5114/ada.2020.94840

Online publish date: 2020/05/06

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AMA

Kolano P, Bednarski IA, Lesiak A, et al. Overexpression of cathepsin K and vascular endothelial growth factor in chronic venous ulcerations. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2020;37(2):234-239. doi:10.5114/ada.2020.94840.

APA

Kolano, P., Bednarski, I. A., Lesiak, A., Skibińska, M., Stasikowska-Kanicka, O.,  & Danilewicz, M. et al. (2020). Overexpression of cathepsin K and vascular endothelial growth factor in chronic venous ulcerations. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 37(2), 234-239. https://doi.org/10.5114/ada.2020.94840

Chicago

Kolano, Paweł, Igor A Bednarski, Aleksandra Lesiak, Małgorzata Skibińska, Olga Stasikowska-Kanicka, Marian Danilewicz,  and Joanna Narbutt. 2020. "Overexpression of cathepsin K and vascular endothelial growth factor in chronic venous ulcerations". Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii 37 (2): 234-239. doi:10.5114/ada.2020.94840.

Harvard

Kolano, P., Bednarski, I., Lesiak, A., Skibińska, M., Stasikowska-Kanicka, O., Danilewicz, M.,  and Narbutt, J. (2020). Overexpression of cathepsin K and vascular endothelial growth factor in chronic venous ulcerations. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 37(2), pp.234-239. https://doi.org/10.5114/ada.2020.94840

MLA

Kolano, Paweł et al. "Overexpression of cathepsin K and vascular endothelial growth factor in chronic venous ulcerations." Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, vol. 37, no. 2, 2020, pp. 234-239. doi:10.5114/ada.2020.94840.

Vancouver

Kolano P, Bednarski I, Lesiak A, Skibińska M, Stasikowska-Kanicka O, Danilewicz M et al. Overexpression of cathepsin K and vascular endothelial growth factor in chronic venous ulcerations. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2020;37(2):234-239. doi:10.5114/ada.2020.94840.

Introduction
Chronic venous disease (CVD) is a disabling condition affecting about 1% to 3% of the general population. Besides varicose veins, CVD can result also in the formation of severe skin lesions, especially venous ulcerations (VU). The exact mechanism of VU is still unknown.

Aim
To evaluate immunoexpression of vascular endothelial growth factor (VEGF) and cathepsin K in healthy individuals and patients with VU.

Material and methods
The study included 12 patients with venous ulcers and 10 healthy individuals who served as controls; both groups were sex- and age-matched. Biopsy samples were obtained from lower leg areas and submitted to histochemical analysis.

Results
There was a significant difference between the study group and the control group in cathepsin K expression (1.007 ±0.3 vs. 0.22 ±0.2, respectively, p < 0.001) and VEGF expression (1.17 ±0.59 vs. 0.27 ±0.19, respectively, p < 0.001). Additionally, the microvessel density (per mm²) differed significantly between the study group and the control group (97.6 ±28.81 vs. 59.32 ±12.71, respectively, p < 0.001). We found no correlation between cathepsin K and microvessel density, and cathepsin K and VEGF in both groups, but there was a significant correlation between microvessel density and VEGF immunoexpression in the study group (r = 0.82, p = 0.002).

Conclusions
Increased immunoexpression of VEGF and cathepsin K suggests that both of these proteins may play a role in VU development.

keywords:

Overexpression of cathepsin K and vascular endothelial growth factor in chronic venous ulcerations

Paweł Kolano 1 , Igor A. Bednarski 2 , Aleksandra Lesiak 2 , Małgorzata Skibińska 2 , Olga Stasikowska-Kanicka 3 , Marian Danilewicz 3 , Joanna Narbutt 2

venous ulceration, immunohistochemistry, cathepsin K

Paweł Kolano

¹

,

Igor A. Bednarski

²

,

Aleksandra Lesiak

²

,

Małgorzata Skibińska

²

,

Olga Stasikowska-Kanicka

³

,

Marian Danilewicz

³

,

Joanna Narbutt

²