eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
2/2017
vol. 98
 
Share:
Share:
abstract:
RESEARCH PAPERS

Overexpression of stress-related genes in Cuscuta campestris in response to host defense reactions

Hamed Rezaei
1
,
Khalil Alamisaeed
1
,
Cobra Moslemkhani
2

  1. Agriculture and Natural Resources University of Ramin, Ahvaz, Iran
  2. Seed and Plant Certification and Registration Institute. Agricultural Research, Education and Extension Organization (AREEO), Teheran, Iran
BioTechnologia vol. 98(2) pp. 131-139 2017
Online publish date: 2017/07/18
View full text Get citation
 
PlumX metrics:
Herb dodder (Cuscuta spp.) is one of the most important parasitic plants that can severely affect crop yields in the world. So far, interactions of this parasitic plant with hosts were not investigated adequately. Here, we conducted a differential expression analyzes and identified a number of genes that were differentially expressed in haustorium tissue compared with the stem of Cuscuta campestris growing on Alfalfa. We obtained 439 cDNA fragments from haustoria (parasite-host connection zone) and stems (25 cm away from connections zones) using the cDNA-AFLP (Amplified Fragment Length Polymorphism) method with eight different primer combinations. Of 439 transcript-derived fragments (TDFs) that were detected, 145 fragments were identified as differentially expressed genes. Five TDF sequences were similar to known functional genes involved in signal transduction, metabolism, respiration, and stress responses. Genes encoding DEAD-box ATP-dependent RNA helicase, potential heme-binding protein, lysine-specific demethylase 5A were selected for qRT-PCR. The qRT-PCR analyzes confirmed the results obtained using cDNA-AFLP. Our findings shed light on the elicitation of dodder defense responses in the connection zone to overcome plant defense reactions.
keywords:

Cuscuta campestris, cDNA-AFLP, haustorium, Alfalfa



Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here