4/2011
vol. 92
abstract:
PERSPECTIVES Transcriptome sequencing:
next generation approach to RNA functional analysis
BioTechnologia vol. 92(4) C pp. 311-319 C 2011
Online publish date: 2014/10/28
PlumX metrics:
The most general definition of transcriptome assumes that it is a complete set of RNA molecules (transcripts)
that are formed in a given organism. In response to exogenous and endogenous stimuli, transcriptome undergoes
constant qualitative and quantitative changes. Monitoring these changes can provide information on how the living
organisms function and deal with the changing environmental conditions. Recently, a new approach, called
RNA sequencing or RNA-Seq has been applied to transcriptome-oriented studies. It is based on the next-generation
sequencing and allows transcript discovery and quantification. This new approach permits identification of
novel transcripts and products of their processing or partial degradation. Since RNA-Seq does not require information
about the sequence of the reference genome, it can be used for transcriptome analysis in any organism.
This feature combined with the high sensitivity and broad dynamic range of RNA-Seq experiments, make them
much more versatile for high-throughput studies as compared to DNA microarray technology.
keywords:
RNA-Seq, transcriptome, next-generation sequencing, isoforms
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