Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes

Ilona Szabłowska-Gadomska; Magdalena Ducher; Magdalena Orzechowska; Joanna Bogusławska-Duch; Magdalena Kowalska; Helena Poławska; Maciej Małecki

doi:10.5114/pjp.2018.79550

eISSN: 2084-9869
ISSN: 1233-9687

Polish Journal of Pathology

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3/2018
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abstract:

Original paper

Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes

Ilona Szabłowska-Gadomska

,

Magdalena Ducher

,

Magdalena Orzechowska

,

Joanna Bogusławska-Duch

,

Magdalena Kowalska

,

Helena Poławska

,

Maciej Małecki

Pol J Pathol 2018; 69 (3): 299-310

DOI: https://doi.org/10.5114/pjp.2018.79550

Online publish date: 2018/11/20

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AMA

Szabłowska-Gadomska I, Ducher M, Orzechowska M, et al. Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes. Polish Journal of Pathology. 2018;69(3):399-310. doi:10.5114/pjp.2018.79550.

APA

Szabłowska-Gadomska, I., Ducher, M., Orzechowska, M., Bogusławska-Duch, J., Kowalska, M.,  & Poławska, H. et al. (2018). Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes. Polish Journal of Pathology, 69(3), 399-310. https://doi.org/10.5114/pjp.2018.79550

Chicago

Szabłowska-Gadomska, Ilona, Magdalena Ducher, Magdalena Orzechowska, Joanna Bogusławska-Duch, Magdalena Kowalska, Helena Poławska,  and Maciej Małecki. 2018. "Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes". Polish Journal of Pathology 69 (3): 399-310. doi:10.5114/pjp.2018.79550.

Harvard

Szabłowska-Gadomska, I., Ducher, M., Orzechowska, M., Bogusławska-Duch, J., Kowalska, M., Poławska, H.,  and Małecki, M. (2018). Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes. Polish Journal of Pathology, 69(3), pp.399-310. https://doi.org/10.5114/pjp.2018.79550

MLA

Szabłowska-Gadomska, Ilona et al. "Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes." Polish Journal of Pathology, vol. 69, no. 3, 2018, pp. 399-310. doi:10.5114/pjp.2018.79550.

Vancouver

Szabłowska-Gadomska I, Ducher M, Orzechowska M, Bogusławska-Duch J, Kowalska M, Poławska H et al. Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes. Polish Journal of Pathology. 2018;69(3):399-310. doi:10.5114/pjp.2018.79550.

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Gynecological cancers constitute a serious problem in the world. Their advanced stages are often characterized by the accumulation of ascites, which leads to spreading of cancer cells outside their primary focus. Despite progress in the treatment, prognoses are still not satisfactory. The main causes of these failures are chemoresistance, metastases and recurrences of the disease, which is influenced by, among others, the microenvironment of cancer cells. This study investigated the effect of the microenvironment, which create ascites derived from patients with ovarian and endometrial cancer to non-gynecological HEK 293 cells. The effect of the gynecological cancer microenvironment on HEK 293 cells behaviour was analysed using RT-PCR, qRT-PCR, Western blotting and functional analysis (invasion assays, hanging drop) methods. Our results suggest that the key genes for the development of cancer can be regulated by epigenetic and hypoxia-inducible factor in dependent manner. It was observed that in vitro microenvironment, which is created by cells originating from patients with gynecological cancer (ovarian cancer, endometrial cancer) is able to generate changes in HEK 293 cells by itself.

keywords:

Peritoneal fluid stimulates neoplastic transformation of normal HEK 293 cells by high expression of pluripotent genes

Ilona Szabłowska-Gadomska , Magdalena Ducher , Magdalena Orzechowska , Joanna Bogusławska-Duch , Magdalena Kowalska , Helena Poławska , Maciej Małecki

microenvironment, ascites, ovarian cancer, endometrial cancer, pluripotency

Ilona Szabłowska-Gadomska

,

Magdalena Ducher

,

Magdalena Orzechowska

,

Joanna Bogusławska-Duch

,

Magdalena Kowalska

,

Helena Poławska

,

Maciej Małecki