eISSN: 1897-4295
ISSN: 1734-9338
Advances in Interventional Cardiology/Postępy w Kardiologii Interwencyjnej
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abstract:
Original paper

Plasma leukocyte-derived extracellular vesicles are related to target vessel revascularization in off-pump coronary artery bypass grafting

Tomasz K. Urbanowicz
1
,
Aleksandra Gąsecka
2
,
Anna Olasińska-Wiśniewska
1
,
Michał Michalak
3
,
Michał Rodzki
1
,
Ewelina Błażejowska
2
,
Krzysztof J. Filipiak
4, 5
,
Mariusz Kowalewski
6, 7
,
Andrzej Tykarski
5
,
Marek Jemielity
1

  1. Cardiac Surgery and Transplantology Department, Poznan University of Medical Sciences, Poznan, Poland
  2. 1st Chair and Department of Cardiology, Medical University of Warsaw, Warsaw, Poland
  3. Department of Computer Science and Statistics, Poznan University of Medical Sciences, Poznan, Poland
  4. Maria Sklodowska-Curie Medical Academy, Warsaw, Poland
  5. Department of Hypertensiology, Angiology and Internal Medicine, Poznan University of Medical Sciences, Poznan, Poland
  6. Department of Cardiac Surgery, Center of Postgraduate Medical Education, Central Clinical Hospital of the Ministry of Interior, Warsaw, Poland
  7. Department of Cardiothoracic Surgery, Heart and Vascular Center, Maastricht University Medical Centre (MUMC), Maastricht, the Netherlands
Adv Interv Cardiol
DOI: https://doi.org/10.5114/aic.2024.145576
Online publish date: 2024/12/02
View full text Get citation
 
Introduction:
In multivessel coronary disease, interventional therapy is recommended to improve symptoms and survival. Inflammatory activation is postulated as one of the coronary atherosclerosis progression risk factors and prognostic indicators of surgical intervention. Counts of plasma extracellular vesicles (EVs) derived from erythrocytes, leukocytes, and platelets are elevated in patients with inflammatory diseases, including atherosclerosis-based cardiovascular diseases.

Aim:
We aimed to estimate the association between blood flow measurements in coronary artery bypass grafts and EV plasma count changes in patients undergoing surgical coronary revascularization.

Material and methods:
Forty-four consecutive patients who underwent surgical off-pump revascularization (33 males, 75%) in the median (Q1–Q3) age of 65 (58–69) were included. The blood flow in the implanted coronary artery bypass grafts was measured intraoperatively. EV counts were measured at baseline (hospital admission) and on the 1st and 3rd postoperative days. The numbers of EVs from erythrocytes (CD235+), leukocytes (CD45+) and platelets (CD61+) were measured using flow cytometry with fluorescently labeled antibodies against specific antigens exposed on the EVs.

Results:
The numbers of leukocyte-derived EVs were lower in patients who received a LIMA-LAD graft (p = 0.020). The total EV plasma count changes were found significant for RIMA-LAD grafting (p = 0.028) and LIMA-LAD (p = 0.015) in arterial and arterio-venous grafting, respectively.

Conclusions:
Surgical revascularizations affect EV plasma count, including significantly lower postoperative values of leukocyte-derived EVs related to left descending artery grafting.

keywords:

left descending artery, off-pump coronary artery bypass grafting, CD61, extracellular vesicles, CD45, CD235
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