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eISSN: 2083-8441
ISSN: 2081-237X
Pediatric Endocrinology Diabetes and Metabolism
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SCImago Journal & Country Rank
2/2023
vol. 29
 
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abstract:
Original paper

Potential bacteriophages to overcome bacterial infection of Alcaligenes faecalis in diabetic ulcer

Erlia Narulita
1, 2
,
Vivi Indah Nur Cahyati
1
,
Riska A. Febrianti
2
,
Mochammad Iqbal
1

  1. Biology Education, Universitas Jember, Indonesia
  2. Laboratory of Molecular Medicine, Universitas Jember, Indonesia
Pediatr Endocrinol Diabetes Metab 2023; 29 (2): 61-66
Online publish date: 2023/03/23
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Introduction
Diabetes is a non-contagious disease, but it can cause various complications. One of the most common complications of diabetes is diabetic ulcers. Diabetic ulcers are infections that occur in the legs of diabetics due to the destruction of the deepest skin tissue. Recent studies have reported the presence of Alcaligenes faecalis with extensive drug resistance (XDR) properties as a cause of diabetic ulcers. Bacteriophages are known to have the ability to infect bacteria specifically so that they can be used as an alternative solution for treating diabetic ulcers. The purpose of this study was to determine the characteristics of bacteriophages capable of infecting Alcaligenes faecalis bacteria.

Material and methods
The method used is the spot test method, host range, and identification of nucleic acid types.

Results
The results showed that the 6 bacteriophages isolated, namely AFaV1, AFaV2, AFaV3, AFaV4, AFaV5, and AFaV6, had cloudy plaques with a diameter of ±3 mm. AFaV1, AFaV2, and AFaV4 isolates could infect all bacteria used; they were Klebsiella pneumoniae, Escherichia coli, and Staphylococcus aureus. Meanwhile, bacteriophage isolates AFaV3, AFaV5, and AFaV6 could infect Klebsiella pneumoniae and Staphylococcus aureus bacteria only. The nucleic acid types of the 6 bacteriophage samples were dsDNA with band length > 1 Kb.

Conclusions
The 6 isolates that were isolated had the ability to infect by forming a prophage that could inhibit the growth of Alcaligenes faecalis and other pathogenic bacteria in diabetic ulcers.

keywords:

Alcaligenes faecalis; bacteriophages; XDR; diabetic ulcer


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