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Polish Journal of Pathology
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2/2020
vol. 71
 
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abstract:
Original paper

Preparation of frozen cell-block sections amenableto diagnostic immunocytochemistry: a technical report on cryo-embedded cell-block method

Joo Sung Lee
1
,
Suk Jin Choi
1, 2
,
Lucia Kim
2
,
In Suh Park
2
,
Jee Young Han
2
,
Joon Mee Kim
2

  1. Department of Pathology, Inha University Hospital, Incheon, Korea
  2. Department of Pathology, Inha University College of Medicine, Incheon, Korea
Pol J Pathol 2020; 71 (2): 146-154
DOI: https://doi.org/10.5114/pjp.2020.97022
Online publish date: 2020/07/21
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Modified agarose cell-block (CB) technique can be effectively used to have the CB embedded in the OCT compound for the preparation of frozen CB (F-CB) sections in the same way as in the preparation of frozen sections from cryoembedded fresh tissue samples for the intraoperative consultation. In this report, we demonstrate the amenability of F-CB sections to the diagnostic immunocytochemistry. The pelleted cytologic material was at first compactly embedded in ultralow-gelling temperature agarose gel and then re-embedded in conventional agarose gel. The resulting agarose-embedded cell-pellet was cut in halves so that one half is embedded in OCT compound for cryosectioning on a cryostat, while the other half is reserved for the preparation of paraffin-embedded CB (P-CB). The F-CB sections were comparable to sections cut from the paraffin-embedded CB in terms of the quality of H&E-staining and immunocytochemistry. We suppose this method can also facilitate a rapid quantitative and qualitative assessment of the future P-CB.

We have extended this technique to the cryo-embedded cell-block method, in which the compact agarose cell pellet is directly embedded in the OCT compound so that the frozen sections can be cut from the cryo-embedded cell block in the same way as in intraoperative frozen section analysis. In this technical report, we illustrate how the frozen cell blocks (F-CBs) can be effectively prepared not only from liquid-based cytology samples but also from conventional fine-needle aspiration cytology slides.
keywords:

cytology, fine needle aspiration, cell-block, frozen section, immunocytochemistry
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