Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet-derived growth factor (PDGF)

Ewa Warnawin; Tomasz Burakowski; Michał Gajewski; Anna Radzikowska; Anna Kornatka; Cezary Michalak; Paweł Małdyk; Sławomir Maśliński; Włodzimierz Maśliński

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures

Editorial System

Submit your Manuscript

1-2/2005
vol. 30

Send email

Copy url:

abstract:

Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet-derived growth factor (PDGF)

Ewa Warnawin

,

Tomasz Burakowski

,

Michał Gajewski

,

Anna Radzikowska

,

Anna Kornatka

,

Cezary Michalak

,

Paweł Małdyk

,

Sławomir Maśliński

,

Włodzimierz Maśliński

Centr Eur J Immunol 2005; 30 (1-2): 26-31

Online publish date: 2006/07/26

View full text Get citation

EndNote

Papers, Reference Manager, RefWorks, Zotero

AMA

Warnawin E, Burakowski T, Gajewski M, et al. Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet-derived growth factor (PDGF). Central European Journal of Immunology. 2005;30(1):1-2.

APA

Warnawin, E., Burakowski, T., Gajewski, M., Radzikowska, A., Kornatka, A.,  & Michalak, C. et al. (2005). Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet-derived growth factor (PDGF). Central European Journal of Immunology, 30(1), 1-2.

Chicago

Warnawin, Ewa, Tomasz Burakowski, Michał Gajewski, Anna Radzikowska, Anna Kornatka, Cezary Michalak,  and Paweł Małdyk et al. 2005. "Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet-derived growth factor (PDGF)". Central European Journal of Immunology 30 (1): 1-2.

Harvard

Warnawin, E., Burakowski, T., Gajewski, M., Radzikowska, A., Kornatka, A., Michalak, C., Małdyk, P., Maśliński, S.,  and Maśliński, W. (2005). Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet-derived growth factor (PDGF). Central European Journal of Immunology, 30(1), pp.1-2.

MLA

Warnawin, Ewa et al. "Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet-derived growth factor (PDGF)." Central European Journal of Immunology, vol. 30, no. 1, 2005, pp. 1-2.

Vancouver

Warnawin E, Burakowski T, Gajewski M, Radzikowska A, Kornatka A, Michalak C et al. Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet-derived growth factor (PDGF). Central European Journal of Immunology. 2005;30(1):1-2.

Mesenchymal stem cells (MSC) exert unique ability to differentiate into several cell lineages including: chondrocytes, osteoblasts, adipocytes and myocytes. These properties place MSC as a very promising source of cells for regenerative medicine. However, the optimal conditions for MSC propagation in vitro that could preserve their ability to differentiate into these lineages have to be elucidated. In the present study we tested the hypotheses that: (i) low oxygen concentration, and (ii) the presence of select growth factors, facilitates MSC proliferation in vitro without affecting their chondrogenic potential. MSC cultured in reduced oxygen (5%) proliferated more rapidly than in normal oxygen concentration (21%). Although all tested cytokines: PDGF-BB, FGF, IL-1b, IL-15 and TNF-a stimulated MSC proliferation in reduced oxygen level, the best effect was observed in the presence of PDGF-BB. MSC cultured in low oxygen tension and in the presence of PDGF-BB retain their ability to differentiate into chondrocytes. Cultured in chondrogenic medium containing ITS, dexamethasone, TGF-β and ascorbic acid, MSC started to synthesize mRNA encoding chondrocyte specific genes: aggrecan and collagen II. Therefore, culture in low oxygen tension in combination with PDGF BB can be used to propagate MSC in vitro without affecting their chondrogenic potential.

keywords:

Preservation of chondrogenic potential of mesenchymal stem cells isolated from osteoarthritic patients during proliferation in response to platelet-derived growth factor (PDGF)

Ewa Warnawin , Tomasz Burakowski , Michał Gajewski , Anna Radzikowska , Anna Kornatka , Cezary Michalak , Paweł Małdyk , Sławomir Maśliński , Włodzimierz Maśliński

mesenchymal stem cells, cytokines in vitro culture

Ewa Warnawin

,

Tomasz Burakowski

,

Michał Gajewski

,

Anna Radzikowska

,

Anna Kornatka

,

Cezary Michalak

,

Paweł Małdyk

,

Sławomir Maśliński

,

Włodzimierz Maśliński