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BioTechnologia
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3/2016
vol. 97
 
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abstract:
SHORT COMMUNICATIONS

Production of an active human AID enzyme in a bacterial system

Lucyna Budzko
1
,
Paulina Jackowiak
1
,
Marek Figlerowicz
1, 2

  1. Institute of Bioorganic Chemistry, Polish Academy of Sciences, Poznań, Poland
  2. Institute of Computing Science, Poznan University of Technology, Poznań, Poland
vol. 97(3) C pp. 235-239 C 2016
DOI: https://doi.org/10.5114/bta.2016.62360
Online publish date: 2016/11/02
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The aim of our invention is to characterize a modified gene of human activation-induced cytidine deaminase (hAID ), a method of modification of hAID gene, a composition showing hAID activity, and a method of preparation of such composition in a bacterial system. In particular, the invention concerns a modified hAID gene for the production of an active enzyme in a bacterial system as well as use of such composition for the analyzes of DNA/RNA amination/deamination, and/or methylation/demethylation.


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