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ISSN: 0860-7796
BioTechnologia
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2/2011
vol. 92
 
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abstract:

RESEARCH PAPER
Embryogenic callus formation by cotyledon and leaf explants of Crambe abyssinica seedlings

Tomasz Furmanek
,
Walentyna Banaś

BioTechnologia vol. 92(2) C pp. 209-213 C 2011
DOI: https://doi.org/10.5114/bta.2011.46535
Online publish date: 2014/10/28
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The study investigates the regeneration capacity of Crambe abyssinica via the callus phase. The conducted experiments

provide an overview of various conditions appropriate for callus development and its subsequent differentiation.

Explants from cotyledons and leaves of sterile 14-day-old seedlings of C. abyssinica cv. Mayer were used.

The primary callus so obtained was subsequently utilised for the observation of embryogenesis. The best callus

formation on the explants was achieved on the agar-solidified MS medium with 0.5 mg ·dm-3 NAA and 0.5 mg ·dm-3

BAP or TDZ. The formation of secondary embryogenic callus was most efficient on MS medium supplemented

with 0.4 mg ·dm-3 NAA and 0.3 mg ·dm-3 of BAP.
keywords:

Crambe abyssinica, in vitro, callus, embryogenesis


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