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ISSN: 0860-7796
BioTechnologia
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1/2011
vol. 92
 
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RESEARCH PAPER
Optimization of activated sludge storage before RNA isolation

Agnieszka Cydzik-Kwiatkowska
,
Marta Wnuk

BioTechnologia vol. 92(1) C pp. 101-105 C 2011
Online publish date: 2014/10/28
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Proper sample storage before RNA isolation is crucial for a reliable assessment of microbial activity in activated

sludge. In our studies, different amounts of activated sludge expressed in mg of total suspended solids (TSS) were

fixed with a constant volume of RNA later RNA Stabilization Reagent (QIAGEN, Germantown, MD, USA) and

stored for 3 or 6 months. The results obtained point out that for the best RNA recovery, 1 mg of activated sludge

TSS should be fixed with 33 μl of the RNA later. It was also observed that a longer storage period (6 vs. 3 months)

of the activated sludge in RNA later lowered RNA recovery by, on average, 25 ± 15% although bacterial diversity

in all samples remained unaltered.
keywords:

activated sludge, RNAlater, RNA isolation



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