4/2012
vol. 93
abstract:
RESEARCH PAPER Phytotests as tools for monitoring the bioremediation process
of soil contaminated with diesel oil
Olga Marchut-Mikołajczyk
,
BioTechnologia vol. 93(4) C pp. 431-439 C 2012
Online publish date: 2014/10/28
PlumX metrics:
The aim of the study was to determine changes in phytotoxicity levels during bioremediation of soil contaminated
with 5% (v/w) diesel oil and find their correlation with the effectiveness of hydrocarbon degradation. Bioremediation
trials were performed using a Gordonia alkanivorans S7 strain. The clean-up process was enhanced through
the addition of dried fungal enzymatic preparations obtained from fungi Phanerohaete chrysosporium and Aspergillus
niger. After 110 days of bioremediation the decrease in soil pollution ranged between 68 and 77% depending
on treatment conditions. Toxicological tests using marker plants revealed significant differences in the phytotoxicity
levels of soil during bioremediation, depending on the treatment variant. The addition of an enzymatic fungal
preparation to soil was found to accelerate the rate of contaminant degradation. The rate of hydrocarbon depletion
in subsequent phases of the remediation process was found to be correlated with the phytotoxicity level. The obtained
results provide evidence that phytotests can be useful tools for monitoring toxicity changes during
bioremediation either mediated only by bacteria or additionally stimulated by fungal enzymatic preparations.
keywords:
Phytotoxicity test, bioremediation, monitoring, diesel oil, fungal enzymatic preparations
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