4/2011
vol. 92
abstract:
RESEARCH PAPER Wild type root cultures of Scutellaria barbata
Agata Wilczańska-Barska
,
Mirosława Krauze-Baranowska
,
BioTechnologia vol. 92(4) C pp. 369-377 C 2011
Online publish date: 2014/10/28
PlumX metrics:
Scutellaria barbata wild type root cultures were grown on liquid media: Gamborg (B5) and Schenk-Hildebrandt
(SH) supplemented with (1 mg/l) indole-3-butyric acid (IBA). For each culture, growth rate and secondary metabolites
production were determined. It was observed that in the metabolic profiles, the dominating compounds were
flavone glycosides – wogonoside, baicalin and phenylethanoid glycoside acteoside. Aglycones namely wogonin,
baicalein and chrysin – were present at low concentrations. On B5 medium, the average productivity of plant material
counted as the sum of flavones was higher (ca. 1300 mg/l) than on SH medium (ca. 999 mg/l). In order to
remove the compounds that cause browning of the medium and inhibit culture growth, especially on B5 IBA 1.0
medium, a number of experiments with the addition of cork pieces, citric acid and Amberlite XAD-4 and XAD-7
resins into the culture media were performed. The best growth of the root culture was achieved on B5 medium
supplemented with – 1.0 mg/l IBA along with the addition of 7 mg/ml of cork and with 10 mg/ml of Amberlite
XAD-4 resin, although concentrations of the analyzed metabolites were lowers than in the control group.
keywords:
Scutellaria barbata, flavones, wild type root cultures
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