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BioTechnologia
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2/2012
vol. 93
 
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abstract:

SHORT COMMUNICATION
The effect of growth regulators on the regeneration ability of flax (Linum usitatissimum L.) hypocotyl explants in in vitro culture

Jowita Janowicz
,
Janetta Niemann
,
Andrzej Wojciechowski

BioTechnologia vol. 93(2) C pp. 135-138 C 2012
DOI: https://doi.org/10.5114/bta.2012.46578
Online publish date: 2014/10/28
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The aim of the present study is to determine the influence of different concentrations of growth regulators on

the hypocotyl explants regeneration abilities of flax cultivars (Linum usitatissimum L.). Plant material used in

the study was obtained from two flax cultivars: Modran and Selena. Hypocotyl explants were obtained from 6-dayold

seedlings and were subsequently placed on basal MS (Murashige and Skoog 1962) medium and MS medium

supplemented with 6-benzylaminopurine (BAP) or naftalene acetic acid (NAA). Final evaluation of the callus,

the shoot and the root formation of the explants was performed 28 days after the experimental setup. The highest

regeneration effectiveness was observed on the media supplemented with 1 mg/l BAP.
keywords:

flax, regeneration, in vitro cultures, hypocotyl, BAP, NAA


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