Selected immunohistochemical features of conventional renal cell carcinomas coexpressing P53 and MDM2

Maria Hejnold; Grzegorz Dyduch; Magdalena Białas; Sergiusz Demczuk; Janusz Ryś; Piotr Chłosta; Tomasz Szopiński; Krzysztof Okoń

doi:10.5114/pjp.2014.43960

eISSN: 2084-9869
ISSN: 1233-9687

Polish Journal of Pathology

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2/2014
vol. 65

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abstract:

Selected immunohistochemical features of conventional renal cell carcinomas coexpressing P53 and MDM2

Maria Hejnold

,

Grzegorz Dyduch

,

Magdalena Białas

,

Sergiusz Demczuk

,

Janusz Ryś

,

Piotr Chłosta

,

Tomasz Szopiński

,

Krzysztof Okoń

Pol J Pathol 2014; 65 (2): 113-119

DOI: https://doi.org/10.5114/pjp.2014.43960

Online publish date: 2014/07/28

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Renal clear cell carcinoma (CCRCC) is an aggressive tumor for which new prognostic factors are needed. It has been suggested that CCRCCs co-expressing P53 and MDM2 could represent a special subgroup; therefore the aim of this study was to explore their immunohistochemical features. The material studied consisted of 470 cases of CCRCC. Immunohistochemistry for MDM2, P53, Ki-67, VEGF-A, VEGF-C, VEGF-D, GLUT1, CA9, and CK 7 was performed on tissue microarrays and assessed semi-quantitatively. On average, 6.6% or 5.3% of cases were P53+/MDM2+, depending on the P53 antibody used. The mean percentage of Ki-67 positive cells was 0.6% and p53-positive MDM2-positive cases showed significantly higher expression of Ki-67. The other immunohistochemical parameters studied did not differ between p53-positive MDM2-positive cases and the rest of the subtypes studied. Expression of almost all immunohistochemical markers differed with respect to pT stage; only for CA9 was the difference not significant. Furthermore, almost all immunohistochemical markers studied differed with respect to differences in grade; only for GLUT1 was the difference not significant. Our results suggest that with the exception of Ki-67, there are no significant associations between analyzed markers and the double P53+/MDM2+ phenotype.

keywords:

Selected immunohistochemical features of conventional renal cell carcinomas coexpressing P53 and MDM2

Maria Hejnold , Grzegorz Dyduch , Magdalena Białas , Sergiusz Demczuk , Janusz Ryś , Piotr Chłosta , Tomasz Szopiński , Krzysztof Okoń

renal cell carcinoma, MDM2, P53, Ki-67, VEGF-A, VEGF-C, VEGF-D, GLUT1, CK 7, CA9

Maria Hejnold

,

Grzegorz Dyduch

,

Magdalena Białas

,

Sergiusz Demczuk

,

Janusz Ryś

,

Piotr Chłosta

,

Tomasz Szopiński

,

Krzysztof Okoń