Sputum interleukin-25 correlates with asthma severity: a preliminary study

Magdalena Paplińska-Goryca; Elżbieta M. Grabczak; Marta Dąbrowska; Joanna Hermanowicz-Salamon; Małgorzata Proboszcz; Patrycja Nejman-Gryz; Marta Maskey-Warzęchowska; Rafał Krenke

doi:10.5114/ada.2017.71428

eISSN: 2299-0046
ISSN: 1642-395X

Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii

Current issue Archive Manuscripts accepted About the journal Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures

Editorial System

Submit your Manuscript

Editorial Policies

Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

5/2018
vol. 35

Send email

Copy url:

abstract:

Original paper

Sputum interleukin-25 correlates with asthma severity: a preliminary study

Magdalena Paplińska-Goryca

,

Elżbieta M. Grabczak

,

Marta Dąbrowska

,

Joanna Hermanowicz-Salamon

,

Małgorzata Proboszcz

,

Patrycja Nejman-Gryz

,

Marta Maskey-Warzęchowska

,

Rafał Krenke

Adv Dermatol Allergol 2018; XXXV (5): 462-469

DOI: https://doi.org/10.5114/ada.2017.71428

Online publish date: 2018/07/19

View full text Get citation

EndNote

Papers, Reference Manager, RefWorks, Zotero

AMA

Paplińska-Goryca M, Grabczak E, Dąbrowska M, et al. Sputum interleukin-25 correlates with asthma severity: a preliminary study. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2018;35(5):462-469. doi:10.5114/ada.2017.71428.

APA

Paplińska-Goryca, M., Grabczak, E., Dąbrowska, M., Hermanowicz-Salamon, J., Proboszcz, M.,  & Nejman-Gryz, P. et al. (2018). Sputum interleukin-25 correlates with asthma severity: a preliminary study. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 35(5), 462-469. https://doi.org/10.5114/ada.2017.71428

Chicago

Paplińska-Goryca, Magdalena, Elżbieta M. Grabczak, Marta Dąbrowska, Joanna Hermanowicz-Salamon, Małgorzata Proboszcz, Patrycja Nejman-Gryz,  and Marta Maskey-Warzęchowska et al. 2018. "Sputum interleukin-25 correlates with asthma severity: a preliminary study". Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii 35 (5): 462-469. doi:10.5114/ada.2017.71428.

Harvard

Paplińska-Goryca, M., Grabczak, E., Dąbrowska, M., Hermanowicz-Salamon, J., Proboszcz, M., Nejman-Gryz, P., Maskey-Warzęchowska, M.,  and Krenke, R. (2018). Sputum interleukin-25 correlates with asthma severity: a preliminary study. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 35(5), pp.462-469. https://doi.org/10.5114/ada.2017.71428

MLA

Paplińska-Goryca, Magdalena et al. "Sputum interleukin-25 correlates with asthma severity: a preliminary study." Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, vol. 35, no. 5, 2018, pp. 462-469. doi:10.5114/ada.2017.71428.

Vancouver

Paplińska-Goryca M, Grabczak E, Dąbrowska M, Hermanowicz-Salamon J, Proboszcz M, Nejman-Gryz P et al. Sputum interleukin-25 correlates with asthma severity: a preliminary study. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2018;35(5):462-469. doi:10.5114/ada.2017.71428.

Introduction
Interleukin 25 is an epithelial-derived cytokine associated with allergic Th2 inflammation. However, little is known about the role of IL-25 in different asthma phenotypes and its relationship with disease severity.

Aim
To evaluate and compare the mRNA and protein expression of IL-25 in patients with mild-to moderate/severe asthma and cough variant asthma (CVA).

Material and Methods
Thirty-eight patients with stable asthma (11 patients with mild-to-moderate asthma, 14 patients with severe asthma and 13 patients with CVA) and 14 control subjects were enrolled. IL-25 protein concentration was measured in induced sputum (IS) supernatants by ELISA and IL-25 mRNA expression was evaluated in IS cells by real time PCR.

Results
No differences in IS IL-25 mRNA and IL-25 concentration between controls and the whole asthma group were found. In the detailed analysis, a lower IL-25 mRNA expression in sputum cells was observed in severe asthma compared to CVA and controls. IL-25 protein concentration in sputum supernatants was elevated in patients with severe asthma compared to controls, CVA and mild-to-moderate asthma. A sputum IL-25 level was increased in atopic vs. non-atopic asthma patients. The elevated IL-25 mRNA expression and protein concentration was associated with a lower eosinophil and higher neutrophil percentage in asthmatic airways.

Conclusions
Our results suggest that IL-25 is particularly associated with severe asthma. The relationship between IL-25 and neutrophilic airway inflammation suggests the pleiotropic role of IL-25 in the immune response in this disease.

keywords:

Sputum interleukin-25 correlates with asthma severity: a preliminary study

Magdalena Paplińska-Goryca , Elżbieta M. Grabczak , Marta Dąbrowska , Joanna Hermanowicz-Salamon , Małgorzata Proboszcz , Patrycja Nejman-Gryz , Marta Maskey-Warzęchowska , Rafał Krenke

interleukin 25, asthma, neutrophils, eosinophils, induced sputum

Magdalena Paplińska-Goryca

,

Elżbieta M. Grabczak

,

Marta Dąbrowska

,

Joanna Hermanowicz-Salamon

,

Małgorzata Proboszcz

,

Patrycja Nejman-Gryz

,

Marta Maskey-Warzęchowska

,

Rafał Krenke