The 5A/6A promoter polymorphism of matrix metalloproteinase 3 (MMP-3) gene in menopausal women with ovarian cancer

Andrzej Kulig; Beata Smolarz; Elżbieta Kozłowska; Hanna Romanowicz-Makowska; Krzysztof Szyłło; Mariusz Niewiadomski; Tomasz Rechberger

eISSN: 2299-0038
ISSN: 1643-8876

Menopause Review/Przegląd Menopauzalny

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3/2002
vol. 1

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abstract:

The 5A/6A promoter polymorphism of matrix metalloproteinase 3 (MMP-3) gene in menopausal women with ovarian cancer

Andrzej Kulig

,

Beata Smolarz

,

Elżbieta Kozłowska

,

Hanna Romanowicz-Makowska

,

Krzysztof Szyłło

,

Mariusz Niewiadomski

,

Tomasz Rechberger

(Prz Menopauz 2002, 3: 21–25)

Online publish date: 2004/03/03

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Background: Matrix metalloproteinases (MMPs) comprise family proteolytic enzymes that degrade of extracellular matrix components and therefore play an important role in tumour cell invasion and cancer metastasis. Overexpression of matrix metalloproteinase 3 (MMP-3 or stromelysin 1) gene has been demonstrated in various types of cancers and high level of MMP-3 protein in tumour is a poor prognostic factor for patients. The insertion (6A)/deletion (5A) polymorphism (5A/6A polymorphism) located at the promoter of the MMP-3 gene may have functional significance in the regulation of its expression.
Materials and methods: In the present work the distribution of genotypes and frequencies of alleles of the 5A/6A polymorphism in subjects with ovarian cancer were investigated. Paraffin embedded tumour tissues were obtained from 50 menopausal women with ovarian cancer. The 5A/6A polymorphism were determined by PCR amplification using the allele specific primers.
Results: The distribution of the genotypes of the 5A/6A polymorphism in both control and patients did not differ significantly (p>0.05) from those predicted by the Hardy-Weinberg distribution. Additionally, there were no significant differences (p >0.05) in genotype distributions and allele frequencies between subgroups assigned to histological stage.
Conclusion: The results suggest that the 5A/6A polymorphism of MMP-3 gene may not be linked with appearance and development of ovarian cancer but further research, conducted on larger population, are needed to clarify this point.

keywords:

The 5A/6A promoter polymorphism of matrix metalloproteinase 3 (MMP-3) gene in menopausal women with ovarian cancer

Andrzej Kulig , Beata Smolarz , Elżbieta Kozłowska , Hanna Romanowicz-Makowska , Krzysztof Szyłło , Mariusz Niewiadomski , Tomasz Rechberger

MMP-3 gene, gene polymorphism, ovarian cancer, menopause, PCR

Andrzej Kulig

,

Beata Smolarz

,

Elżbieta Kozłowska

,

Hanna Romanowicz-Makowska

,

Krzysztof Szyłło

,

Mariusz Niewiadomski

,

Tomasz Rechberger