The culture of keratinocytes and epidermal stem cells and their possible application
in the treatment of chronic wounds

Michał Pikuła; Beata Imko-Walczuk; Danuta Nowacka-Pikuła; Aleksandra Okuniewska; Paulina Langa; Janusz Jaśkiewicz; Piotr Trzonkowski

eISSN: 2084-9893
ISSN: 0033-2526

Dermatology Review/Przegląd Dermatologiczny

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3/2012
vol. 99

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abstract:

Original paper

The culture of keratinocytes and epidermal stem cells and their possible application in the treatment of chronic wounds

Michał Pikuła

,

Beata Imko-Walczuk

,

Danuta Nowacka-Pikuła

,

Aleksandra Okuniewska

,

Paulina Langa

,

Janusz Jaśkiewicz

,

Piotr Trzonkowski

Przegl Dermatol 2012, 99, 222–229

Online publish date: 2012/06/25

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Treatment of chronic wounds is still a serious problem in contemporary medicine. In the majority of cases it requires multi-disciplinary cooperation of specialized doctor and nursing teams and significant financial expenses. In many cases the conventional treatment of chronic wounds does not bring the expected therapeutic results, although new alternative therapeutic methods are being researched. One such method is the transplantation of in vitro cultured keratinocytes. The presented work focuses on methods of keratinocyte and epidermal stem cell culture, and the possibility of their transplantation. Safety aspects as well as the limitations of the analysed techniques are discussed. Also the authors present their experience concerning with epidermal cell culture for therapeutic purposes.

keywords:

The culture of keratinocytes and epidermal stem cells and their possible application in the treatment of chronic wounds

Michał Pikuła , Beata Imko-Walczuk , Danuta Nowacka-Pikuła , Aleksandra Okuniewska , Paulina Langa , Janusz Jaśkiewicz , Piotr Trzonkowski

chronic wounds, keratinocyte culture, epidermal stem cells

Michał Pikuła

,

Beata Imko-Walczuk

,

Danuta Nowacka-Pikuła

,

Aleksandra Okuniewska

,

Paulina Langa

,

Janusz Jaśkiewicz

,

Piotr Trzonkowski