The effect of immunonutrition on bacterial translocation after Pringle maneuverer in rats

Ozkan Subasi; Selim Yildiz; Adem Yuksel; Murat Coskun; Hamdi Turgut; Eda Yildiz

doi:10.5114/pg.2019.88166

eISSN: 1897-4317
ISSN: 1895-5770

Gastroenterology Review/Przegląd Gastroenterologiczny

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3/2019
vol. 14

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abstract:

Original paper

The effect of immunonutrition on bacterial translocation after Pringle maneuverer in rats

Ozkan Subasi

¹

,

Selim Yigit Yildiz

¹

,

Adem Yuksel

²

,

Murat Coskun

¹

,

Hamdi Taner Turgut

¹

,

Eda Yildiz

³

Department of General Surgery, Kocaeli Derince Training and Research Hospital, Kocaeli, Turkey
Department of Gastrointestinal Surgery, Kocaeli Derince Training and Research Hospital, Kocaeli, Turkey
Department of Clinical Microbiology, Kocaeli Derince Training and Research Hospital, Kocaeli, Turkey

Gastroenterology Rev 2019; 14 (3): 178–182

DOI: https://doi.org/10.5114/pg.2019.88166

Online publish date: 2019/09/27

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Introduction
Temporary clamping of the hepatoduodenal ligament (the Pringle manoeuvre) is the most commonly used method to prevent intraoperative blood loss in liver surgery. Some side effects of the Pringle manoeuvre (PM) can occur.

Aim
To investigate the effectiveness of preoperative immunonutritional support to prevent bacterial translocation (BT) in rats due to PM.

Material and methods
Forty Wistar albino rats were randomly divided into four groups. Groups 1 and 2 were fed with normal rat diet and water, and groups 3 and 4 were fed with enteral immunonutrition (Impact Glutamine) containing 1 g/kg/day amino acid in the preoperative period. Group 1 (n = 10) and 4 (n = 10) rats were treated only with laparotomy; group 2 (n = 10) and 3 (n = 10) rats were treated with PM for 30 min with laparotomy. After 30 min, relaparotomy was applied to all groups and portal blood, mesentery, spleen samples were taken for culture purposes.

Results
Proliferation in portal blood cultures was significantly higher in the samples from the normally fed group (group 2) in whom PM was applied, compared to the other groups (p < 0.001). No proliferation was observed in the PM-treated group (group 3), who also received preoperative immunonutritional support.

Conclusions
Preoperative immunonutritional support is effective in the prevention of BT due to PM in rats.

keywords:

The effect of immunonutrition on bacterial translocation after Pringle maneuverer in rats

Ozkan Subasi 1 , Selim Yigit Yildiz 1 , Adem Yuksel 2 , Murat Coskun 1 , Hamdi Taner Turgut 1 , Eda Yildiz 3

immunonutrition, Pringle manoeuvre, bacterial translocation

Ozkan Subasi

¹

,

Selim Yigit Yildiz

¹

,

Adem Yuksel

²

,

Murat Coskun

¹

,

Hamdi Taner Turgut

¹

,

Eda Yildiz

³