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4/5/2004
vol. 106 abstract:
Experimental research
The expression of Bcl-2, Bcl-xl, Bak and Bax proteins in axons of the optic nerve in absolute glaucoma
Renata Zalewska
1
,
Joanna Reszeć
2
,
Zofia Mariak
1
,
Stanisław Sulkowski
2
Online publish date: 2004/08/21
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Purpose: The aim of our study was the evaluation of Bcl-2, Bcl-xl, Bak and Bax immunoexpression in axons of the optic nerve
with absolute glaucoma. Material and methods: This study was conducted on 19 patients with absolute glaucoma, suffered from severe ophthalmalgia, treated of necessity by enucleation in 1991-2003 in our Department of Ophthalmology. The samples were immuno-stained with antibodies for Bcl-2, Bak, Bax and Bcl-xl protein. The reactions were performed in Labelled Streptavidin Avidin Biotin (LSAB) technique. The evaluation of immuno-staining was calculated as the percentage of cells with positive reaction to the total number of cells encountered in 10 representative fields, by light microscopy using a x 20 objective lens. Scores were based on the following scale: (++), over 50% of the field showing positive immuno-staining, (+) 10-50% with positive immunostaining. Results: In our study proapoptotic Bak and Bax protein expression was stronger than antiapoptotic Bcl-2, Bcl-xl protein expression; Bak/Bcl-2 (p = 0,008), Bax/Bcl-2 (p = 0,0007), Bak/Bcl-xl (p = 0,0356), Bax/Bcl-xl (p = 0,0077). Conclusions: The results of our study demonstrate high proapoptotic trends in axons of the optic nerve in the stage of absolute glaucoma. keywords:
axons, the optic nerve, glaucoma, apoptosis, Bcl-2, Bcl-xl, Bax, Bak |
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