twitter
en POLSKI
eISSN: 2719-3209
ISSN: 0023-2157
Klinika Oczna / Acta Ophthalmologica Polonica
Current issue Archive Videos Articles in press About the journal Supplements Editorial board Reviewers Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
4/5/2004
vol. 106
 
Share:
Share:
abstract:
Experimental research

The expression of Bcl-2, Bcl-xl, Bak and Bax proteins in axons of the optic nerve in absolute glaucoma

Re­na­ta Za­lew­ska
1
,
Jo­an­na Re­szeć
2
,
Zofia Mariak
1
,
Sta­nisław Sul­kow­ski
2

  1. Z Kliniki Okulistyki Akademii Medycznej w Białymstoku
  2. Zakład Patomorfologii Akademii Medycznej w Białymstoku
Online publish date: 2004/08/21
View full text Get citation
 
Purpose: The aim of our study was the evaluation of Bcl-2, Bcl-xl, Bak and Bax immunoexpression in axons of the optic nerve

with absolute glaucoma.

Material and methods: This study was conducted on 19 patients with absolute glaucoma, suffered from severe ophthalmalgia,

treated of necessity by enucleation in 1991-2003 in our Department of Ophthalmology. The samples were immuno-stained with

antibodies for Bcl-2, Bak, Bax and Bcl-xl protein. The reactions were performed in Labelled Streptavidin Avidin Biotin (LSAB)

technique. The evaluation of immuno-staining was calculated as the percentage of cells with positive reaction to the total

number of cells encountered in 10 representative fields, by light microscopy using a x 20 objective lens. Scores were based on

the following scale: (++), over 50% of the field showing positive immuno-staining, (+) 10-50% with positive immunostaining.

Results: In our study proapoptotic Bak and Bax protein expression was stronger than antiapoptotic Bcl-2, Bcl-xl protein

expression; Bak/Bcl-2 (p = 0,008), Bax/Bcl-2 (p = 0,0007), Bak/Bcl-xl (p = 0,0356), Bax/Bcl-xl (p = 0,0077).

Conclusions: The results of our study demonstrate high proapoptotic trends in axons of the optic nerve in the stage of absolute

glaucoma.
keywords:

axons, the optic nerve, glaucoma, apoptosis, Bcl-2, Bcl-xl, Bax, Bak

Quick links
© 2024 Termedia Sp. z o.o.
Developed by Bentus.