The immunopotentiating effect of Listeriolysin O in the response against Leishmania major in BALB/c mice

Jawaher A. Al-Salem; Khaled S. Tabbara

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures

Editorial System

Submit your Manuscript

3/2003
vol. 28

Send email

Copy url:

abstract:

The immunopotentiating effect of Listeriolysin O in the response against Leishmania major in BALB/c mice

Jawaher A. Al-Salem

,

Khaled S. Tabbara

Centr Eur J Immunol 2003; 28 (3): 119–125

Online publish date: 2004/04/29

View full text Get citation

The development of a Leishmania major protective Th1 response is dependent on IL-12 production. Susceptible BALB/c mice develop a Th2 response. We have previously demonstrated that coinfection with Listeria monocytogenes resulting in type 1 cytokine production (IL-12 and IFN-g), potentiated the response of BALB/c mice to L. major. We investigated here whether Listeriolysin O (LLO), essential for the development of a protective anti-listerial response, was also required to immunopotentiate the response against L. major. Coinfection with L. major and an LLO-producing (LLO+), but not an LLO-nonproducing (LLO–) L. monocytogenes strain, reduced lesion size and parasite load, and increased IFN-g production. Semi-purified LLO also potentiated the response as shown by the containment of parasites in the spleens in well-defined focal granulomas in contrast to controls where more mature disorganized diffuse granulomas with higher parasite loads and necrosis were commonly seen.

keywords:

The immunopotentiating effect of Listeriolysin O in the response against Leishmania major in BALB/c mice

Jawaher A. Al-Salem , Khaled S. Tabbara

Leishmania, Listeria, coinfection, Listeriolysin O, IFN-g, Th1 cells

Jawaher A. Al-Salem

,

Khaled S. Tabbara