Imbalanced Th17/Treg in peripheral blood
of adult patients with immunoglobulin A
vasculitis nephritis

Xue Xue; Qiang Liu; Wencheng Xu; Jun Yuan; Huimin Zhou; Xinrong Zou; Siping Han; Xiangyan Meng; Xiaoqin Wang

doi:10.5114/ceji.2021.108176

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2021
vol. 46

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abstract:

Clinical immunology

Imbalanced Th17/Treg in peripheral blood of adult patients with immunoglobulin A vasculitis nephritis

Xue Xue

¹

,

Qiang Liu

^{2, 3}

,

Wencheng Xu

^{2, 3}

,

Jun Yuan

¹

,

Huimin Zhou

⁴

,

Xinrong Zou

^{2, 3}

,

Siping Han

^{2, 3}

,

Xiangyan Meng

¹

,

Xiaoqin Wang

^{2, 3}

Department of Internal Medicine, The First Clinical College, Hubei University of Traditional Chinese Medicine, Wuhan, Hubei, China
Hubei Provincial Hospital of Traditional Chinese Medicine, Wuhan, Hubei, China
Hubei Province Academy of Traditional Chinese Medicine, Wuhan, Hubei, China
School of Nursing, Hubei University of Traditional Chinese Medicine, Wuhan, Hubei, China

Cent Eur J Immunol 2021; 46 (2): 191-198

DOI: https://doi.org/10.5114/ceji.2021.108176

Online publish date: 2021/08/07

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Introduction
Adult immunoglobulin A vasculitis nephritis (IgAVN) was observed to be more severe than the disease in children because it tended to result in a poor prognosis. The present study analyzed the Th17/Treg cell axis in peripheral blood of adult IgAVN patients, aiming to provide new immunological viewpoints for the pathogenesis of adult IgAVN.

Material and methods
Th17 cell and Treg cell frequencies in peripheral blood of healthy subjects (n = 13) and adult IgAVN patients (n = 12) were analyzed by flow cytometry. Foxp3 mRNA in peripheral blood of healthy subjects and adult IgAVN patients was detected by RT-PCR. Interleukin (IL)-17 and IL-10 in peripheral blood serum of healthy subjects and adult IgAVN patients were examined by ELISA.

Results
The percentages of CD4+ Th17+ cells in peripheral blood of healthy subjects and adult IgAVN patients were 2.65 ±1.55% and 4.37 ±1.68% respectively. The percentages of Treg cells in peripheral blood of healthy subjects and adult IgAVN patients were 6.44 ±2.90% and 3.91 ±1.94% respectively. The ratio of Th17/Treg in adult IgAVN patients was significantly higher than that of healthy subjects (p = 0.0030). Meanwhile, the Foxp3 mRNA expression of adult IgAVN patients was significantly lower than that of healthy subjects. There was a significant difference in the ratio of IL-17/IL-10 between healthy subjects and adult IgAVN patients (p < 0.0001). A significant correlation between red blood cell distribution width (RDW) and the ratio of Th17/Treg in adult IgAVN patients was observed in Spearman correlation analysis (r = 0.6970, p = 0.0145).

Conclusions
Imbalanced Th17/Treg contributed to the complex pathogenesis of adult IgAVN.

keywords:

Imbalanced Th17/Treg in peripheral blood of adult patients with immunoglobulin A vasculitis nephritis

Xue Xue 1 , Qiang Liu 2, 3 , Wencheng Xu 2, 3 , Jun Yuan 1 , Huimin Zhou 4 , Xinrong Zou 2, 3 , Siping Han 2, 3 , Xiangyan Meng 1 , Xiaoqin Wang 2, 3

adult, immunoglobulin A vasculitis nephritis, red blood cell distribution width, Th17, Treg

Xue Xue

¹

,

Qiang Liu

^{2, 3}

,

Wencheng Xu

^{2, 3}

,

Jun Yuan

¹

,

Huimin Zhou

⁴

,

Xinrong Zou

^{2, 3}

,

Siping Han

^{2, 3}

,

Xiangyan Meng

¹

,

Xiaoqin Wang

^{2, 3}