Alzheimer’s amyloid-β peptide disturbs P2X7 receptor-mediated circadian oscillations of intracellular calcium

Anna Wilkaniec; Karen Schmitt; Amandine Grimm; Joanna B. Strosznajder; Anne Eckert

doi:10.5114/fn.2016.64813

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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4/2016
vol. 54

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abstract:

Original paper

Alzheimer’s amyloid-β peptide disturbs P2X7 receptor-mediated circadian oscillations of intracellular calcium

Anna Wilkaniec

^{1, 2, 3}

,

Karen Schmitt

^{1, 2}

,

Amandine Grimm

^{1, 2}

,

Joanna B. Strosznajder

³

,

Anne Eckert

^{1, 2}

Neurobiology Laboratory for Brain Aging and Mental Health, Transfaculty Research Platform, Molecular and Cognitive Neuroscience, University of Basel, Basel, Switzerland,
Psychiatric University Clinics, University of Basel, Basel, Switzerland
Mossakowski Medical Research Center, Polish Academy of Sciences, Department of Cellular Signaling, Warsaw, Poland

Folia Neuropathol 2016; 54 (4): 360-368

DOI: https://doi.org/10.5114/fn.2016.64813

Online publish date: 2016/12/27

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Recent data indicate that Alzheimer’s disease (AD) is associated with disturbances of the circadian rhythm in patients. We examined the effect of amyloid-β (Aβ) peptide, the main component of the senile plaques playing a critical role in the deregulation of calcium (Ca²⁺) homeostasis in AD, on the circadian oscillation of cytosolic calcium (Ca²⁺) levels in vitro. The experiments we carried out in human primary skin fibroblasts. This cell line was previously shown to exhibit circadian rhythms of clock genes. Moreover, the basic clock properties of these peripheral cells closely mimic those measured physiologically and behaviorally in human and do not change during aging. In this study we showed that i) cytosolic Ca²⁺ oscillations depend on the activation of purinergic P2X7 receptors; and ii) these oscillations are abolished in the presence of Aβ. In total, our new findings may help to deepen our understanding of the molecular mechanisms involved in AD-related circadian alterations.

keywords:

Alzheimer’s amyloid-β peptide disturbs P2X7 receptor-mediated circadian oscillations of intracellular calcium

Anna Wilkaniec 1, 2, 3 , Karen Schmitt 1, 2 , Amandine Grimm 1, 2 , Joanna B. Strosznajder 3 , Anne Eckert 1, 2

amyloid-β, Alzheimer’s disease, calcium, P2X7 receptors, circadian rhythms

Anna Wilkaniec

^{1, 2, 3}

,

Karen Schmitt

^{1, 2}

,

Amandine Grimm

^{1, 2}

,

Joanna B. Strosznajder

³

,

Anne Eckert

^{1, 2}