Introduction
Surgery is a frequent cause of secondary thrombocytopenia [1]. In cardiac surgery, hemodilutional and the destructive effect of the cardiopulmonary bypass circuit on thrombocyte count could lead to more than a 50% decrease in platelet count compared to pre-cardiopulmonary bypass values [2]. Typically, the lowest platelet count is found to be 40% to 60% of the preoperative blood thrombocyte level and is detected on the postoperative second or third day [3]. In 10% of patients, more than a 50% reduction in thrombocyte count in comparison to preoperative levels is observed on the fourth postoperative day [4]. Furthermore, preoperative use of antiplatelet agents, prolonged cardio-pulmonary bypass (CPB) time, and hypothermia aggravate thrombocyte dysfunction. Plasmin also contributes to platelet dysfunction. On the other hand, persistent bleeding can add to coagulation dysfunction by exhausting coagulation factors and platelets [5, 6]. In conclusion, cardiac surgery is a process prolonging the postoperative bleeding time by affecting platelets both quantitatively and qualitatively. Regarding aortic valve surgery, valve replacement is the most commonly used procedure despite the presence of multiple aortic valve repair procedures. The prostheses used in heart valve replacement are grouped into two main types, mechanical and bioprosthesis. Today, in spite of technological and surgical developments, the rate of such complications related to prosthetic valves as bleeding, systemic embolization, valve thrombosis, infection, hemolysis and anticoagulation has decreased but the ideal valve has not been obtained yet. Long-term durability of the mechanical prosthesis is a significant advantage over the bioprosthesis. However, the necessity of lifelong warfarin use in this valve group is disadvantageous compared to the bioprosthesis valve group. On the other hand, common problems of bioprosthetic valves are early degeneration and infection [7]. The use of bioprosthetic valves is always a priority in elderly patients due to the low expectation of life, and the increased risk of hemorrhage or thrombosis due to impaired awareness of warfarin use [8–10].
Aortic valve surgery is a complicated procedure and multidisciplinary follow-up should be performed preoperatively, peroperatively and postoperatively. As in other fields of surgery, the problem of bleeding is the most important factor in increasing mortality and morbidity in aortic valve surgery. The most important parameter for the bleeding and coagulation system is the amount of platelets and their functions. Since platelet activation is known to be a side effect of the cardiac surgery, a number of analyses have been performed to identify the relationship between postoperative thrombocytopenia and aortic valve replacement (AVR). Thrombocytopenia must have a platelet count below 50,000/mm3 in order to cause serious bleeding complications [11]. After a usual procedure employing cardiopulmonary bypass, the thrombocyte count remains over 100,000/mm3 despite a reduction of 30% to 50%. Although the bleeding time nearly doubles after CPB, it returns to a normal level within 4 to 12 hours.
Thrombocytopenia developed in the postoperative period after biological aortic valve replacement is observed to have a significantly different pattern and intensity compared to the one seen after CABG and mechanical AVR operations performed under cardiopulmonary bypass.
Aim
In this study, we aimed to investigate the causes of thrombocytopenia after biological AVR and to investigate whether thrombocytopenia is an additional effect in increasing postoperative bleeding or complications.
Material and methods
Patients
We retrospectively evaluated 297 patients who underwent biological AVR, CABG and mechanical AVR between January 2013 and September 2017 in our clinic. In the study, bioprosthetic AVR patients, mechanical prosthetic AVR patients, and CABG patients were defined as group 1, group 2, and group 3, respectively. Patients who underwent double-valve surgery or reoperation, and those with active endocarditis, were excluded from the study. Preoperative and postoperative first 14-day thrombocyte levels of the patients were analyzed. Additionally, patients were analyzed with respect to postoperative bleeding complications and need for renal replacement therapy. Information regarding the gender, age, and thrombocyte count of patients, type of operation done, type and size of prosthesis used, postoperative intra-aortic balloon pump and hemodialysis requirement, and postoperative bleeding complication that required intervention was determined and listed. Daily thrombocyte levels of the patients in whom on at least one of the postoperative first three days the platelet counts were 70 and below were recorded. Patients were classified as having a thrombocyte level of 1–49 (1000/mm3), 50–149 (1000/mm3), and 150 (1000/mm3) or more in terms of platelet counts (Tables I–III). The Sorin Mitroflow aortic pericardial heart valve was preferred as a bioprosthetic valve while the Sorin Carbomedics bileaflet aortic prosthetic heart valve was preferred as a mechanical prosthesis.
Table I
Distribution of descriptive properties
Table II
Distributions of PLT measurements
Table III
Distribution of IABP requirement, hemodialysis requirement and postoperative intervention due to bleeding according to type of surgery
Surgical technique
Under general anesthesia, following median sternotomy, cardiopulmonary bypass was instituted with moderate hypothermia (32–34°C). During AVR operations, the aorta was partially transected and the cardioplegia delivery was done directly through the coronary ostia and repeated at 20 minutes intervals. Aortic valve leaflets and calcifications were removed. Biological prostheses were washed for a sufficient period of time until the glutaraldehyde was thought to be completely cleared. In accordance with the manufacturer’s recommendations valves were rinsed with normal saline at 18–20°C for 3 minutes before implantation and then the prosthesis was implanted. In patients who underwent coronary artery bypass grafting, the left internal mammary artery was preferred for the revascularization of the left anterior descending artery while the great saphenous vein was used in revascularization of other coronary arteries. In coronary artery bypass grafting (CABG), distal anastomoses were performed under the cross clamp, and proximal anastomoses under the side clamp. Patients were taken to the intensive care unit after the operation.
Anticoagulation protocol
In patients who underwent coronary artery bypass surgery, medical treatment of acetylsalicylic acid (300 mg/day p.o.) and enoxaparin (2 × 1 mg/kg/day s.c.) was started on the postoperative first day. All patients who underwent valve surgery were put under enoxaparin (2 × 1 mg/kg/day s.c.) therapy starting from the postoperative first day with warfarin being added on the postoperative second day. On the following days, the warfarin dose was adjusted so that the INR value would be 2–2.5 times the normal. In patients with a bioprosthetic valve use of warfarin was discontinued at the 6th month of follow-up unless there was atrial fibrillation and acetylsalicylic acid (100 mg/day p.o.) therapy was started. Patients with mechanical prostheses were put under lifelong warfarin therapy with INR controls at certain intervals.
Statistical analysis
The NCSS (Number Cruncher Statistical System) 2007 (Kaysville, Utah, USA) program was used for statistical analysis. Student’s t test was used for two-group comparisons of normal distributions in the comparison of quantitative data, and the Mann-Whitney U test was used for two-group comparisons of non-normal distributions beside the descriptive statistical methods (mean, standard deviation, median, frequency, rate, minimum, and maximum). One-way ANOVA test was used for the comparison of three groups with normal distribution, and the Kruskal-Wallis test was used for three groups with non-normal distribution. Age, gender and group effects on platelet values < 150 (1000/mm3) were examined by enter logistic regression analysis. Pearson correlation analysis and Spearman’s correlation analysis were used in evaluating inter-variable relationships. Pearson χ2 test, Fisher-Freeman-Halton test and Fisher’s exact test were used for comparison of qualitative data. The effects of risk factors on platelet values below 150 (1000/mm3) were assessed by multivariate logistic regression analysis. The significance level was p < 0.05.
Results
Of the cases, 28.3% (n = 84) were female and 71.7% were male (n = 213). The ages of the patients ranged from 17 to 88 years with a mean age of 63.11 ±13.33 years (Table I).
The lowest postoperative platelet measurements obtained at follow-ups ranged from 10 to 320 (1000/mm3), with a mean of 118.02 ±49.23 (1000/mm3). The time to reach the lowest postoperative platelet measurement ranged from 0 to 14 days with an average of 1.70 ±1.65 days.
Thrombocytopenia was observed in 96.9% of patients who underwent bioprosthetic AVR. This percentage was 72% in patients with mechanical prosthetic AVR patients. The incidence of thrombocytopenia in the bioprosthetic AVR group was significantly higher than the mechanical prosthetic AVR (p = 0.001) and CABG (p = 0.001) groups (p < 0.01). Severe thrombocytopenia (0–50 × 103/mm3) was observed in 21.6% of patients using a bioprosthesis. However, severe thrombocytopenia was not observed in patients who underwent mechanical prosthetic AVR and CABG. Patients who underwent bioprosthetic AVR had the lowest platelet counts after an average of 2 days. In comparison of the difference between the preoperative and the lowest postoperative platelet values, the difference observed in the bioprosthetic AVR group was found to be significantly larger than that in the mechanical prosthetic AVR group (p = 0.001) and CABG group (p = 0.001) (p < 0.01).
The lowest postoperative platelet measurements according to the type of surgery showed a statistically significant difference (p = 0.001, p < 0.01). As a result of the binary comparisons to determine which group the significance originates from, the measurements of the bioprosthetic AVR group were found to be significantly lower than those of the mechanical prosthetic AVR group (p = 0.001) and CABG group (p = 0.001). The lowest postoperative platelet measurements of the mechanical prosthetic AVR group were statistically significantly lower than those of the CABG group (p = 0.040, p < 0.05).
The rate of platelet measurements in the range of 1–49 (1000/mm3) in the bioprosthetic AVR group was significantly higher than the mechanical prosthetic AVR and CABG groups (p = 0.001; p < 0.01). Similarly, the incidence of falling of platelet levels below 150 (1000/mm3) at any given time shows a statistically significant difference according to the type of operation (p = 0.001, p < 0.01). Binary comparisons to determine which group the significance originates from revealed that the proportion of platelet measurements below 150 (1000/mm3) in the biological valve group was significantly higher than that in the mechanical prosthetic AVR group (p = 0.001) and CABG group (p = 0.001). In this respect, no statistically significant difference was found between the mechanical prosthetic AVR group and CABG group (p > 0.05).
Statistically significant differences were found between platelet levels according to the type of operation (p = 0.001; p < 0.01). In the bioprosthetic AVR group, the percentage of the platelet counts in the range of 1–49 (1000/mm3) was significantly higher than in the mechanical prosthetic AVR and CABG groups (p = 0.001, p < 0.01). The percentage of the platelet counts in the range of 50–99 (1000/mm3) in the bioprosthetic AVR group was significantly higher than those in the mechanical prosthetic AVR and CABG groups. In the mechanical prosthetic AVR group, the percentage of the platelet counts in the range of 50–99 (1000/mm3) was significantly higher than in the CABG group (p = 0.001, p < 0.01). The percentage of the platelet counts in the range of 100–149 (1000/mm3) in the bioprosthetic AVR group was significantly lower than those in the mechanical prosthetic AVR and CABG groups (p = 0.001, p < 0.01). In the bioprosthetic AVR group, the percentage of the platelet measurements over 150 (1000/mm3) was significantly lower than in the mechanical prosthesis AVR and CABG groups (p = 0.001, p < 0.01).
The differences between the preoperative platelet measurements and the lowest postoperative platelet measurements were statistically significant with regard to type of surgery (p = 0.001; p < 0.01). Binary comparisons to determine which group the significance originates from revealed that the differences in the bioprosthetic AVR group were significantly larger than those of the mechanical prosthetic AVR (p = 0.001) and CABG (p = 0.001) groups (p < 0.01). In this respect, there was no statistically significant difference between mechanical prosthetic AVR and CABG groups (p > 0.05). Average prosthesis size of the patients whose platelet measurements at any time were found to be below 150 (1000/mm3) was 22.11 ±1.33 mm, whereas that of the cases having a platelet count of 150 (10000/mm3) or more was 22.33 ±2.31 mm (Table IV).
Table IV
Evaluation of platelet measurements according to type of surgery
| Parameter | Type of surgery | P-value | |||
|---|---|---|---|---|---|
| Bioprosthetic AVR (n = 97) | Mechanical prosthetic AVR (n = 100) | CABG (n = 100) | |||
| Minimum platelet count[× 103/mm3] | Min.–max. (median) | 10–182 (72) | 55–320 (130) | 77–283 (141.5) | a0.001** |
| Mean ± SD | 76.99 ±35.02 | 130.93 ±43.53 | 144.92 ±40.16 | ||
| Time to reach the lowest postoperative platelet count [days] | Min.–max. (median) | 0–14 (2) | 0–4 (1) | 0–14 (1) | b0.001** |
| Mean ± SD | 2.37 ±1.79 | 1.41 ±1.12 | 1.35 ±1.77 | ||
| Platelet levels, n (%) | < 150 | 94 (96.9) | 72 (72.0) | 60 (60.0) | c0.001** |
| ≥ 150 | 3 (3.1) | 28 (28.0) | 40 (40.0) | ||
| 1–49 | 21 (21.6) | 0 (0) | 0 (0) | c0.001** | |
| 50–99 | 51 (52.6) | 25 (25.0) | 13 (13.0) | c0.001** | |
| 100–149 | 22 (22.7) | 47 (47.0) | 47 (47.0) | c0.001** | |
| ≥ 150 | 3 (3.1) | 28 (28.0) | 40 (40.0) | c0.001** | |
| Difference betweenthe preoperative andthe lowest postoperative platelet counts [× 103/mm3] | Min.–max. (median) | 21–385 (139) | 7–248 (91) | 2–222 (85) | b0.001** |
| Mean ± SD | 146.08 ±58.12 | 95.61 ±45.62 | 85.57 ±45.42 | ||
The rate of the platelet level being lower than 150 (1000/mm3) at least once in any measurement was found to be 76.1% (n = 226), while the rate of the platelet level being 150 (1000/mm3) or more was 23.9% (n = 71). Platelet levels ranged from 1 to 49 (1000/mm3) in 7.1% of cases (n = 21); between 50 and 99 (1000/mm3) in 30.0% (n = 89); between 100 and 149 (1000/mm3) in 39.1% (n = 116); and 150 (1000/mm3) or more in 23.9% (n = 71). The differences between the preoperative and the postoperative lowest platelet (PLT) measurements ranged from 2 to 385 (1000/mm3) with a mean of 108.71 ±56.37 (1000/mm3) (Table V).
Table V
Distributions related to platelet measurements
There was no statistically significant difference between thrombocyte levels with regard to the sizes of bioprosthetic valves used (p > 0.05) (Table VI).
Table VI
Assessment of platelet levels according to prosthesis sizes in bioprosthetic AVR group (n = 97)
| Plateletlevels | N | Size | P-value | |
|---|---|---|---|---|
| Min.–max. (median) | Mean ± SD | |||
| < 150 | 94 | 19–27 (23) | 22.11 ±1.33 | – |
| ≥ 150 | 3 | 21–25 (21) | 22.33 ±2.31 | |
| 1–49 | 21 | 21–25 (23) | 22.24 ±1.18 | a0.610 |
| 50–99 | 51 | 19–25 (21) | 21.98 ±1.29 | |
| 100–149 | 22 | 21–27 (22) | 22.27 ±1.58 | |
| ≥ 150 | 3 | 21–25 (21) | 22.33 ±2.31 | |
There was no statistically significant difference in terms of intervention due to bleeding, intra-aortic balloon pump (IABP) or hemodialysis requirement in the three groups (Table III).
In all of the women 100% (n = 40) and 94.7% of the males (n = 54) platelet levels were found to be below 150 (1000/mm3) at least once in any measurement. Platelet levels falling below 150 (1000/mmł) did not show a statistically significant difference according to gender. There was no significant difference in age and gender in the mechanical AVR and CABG groups (p > 0.05) (Table VII).
Table VII
Distribution and statistical analysis of the descriptive characteristics of the cases
| Parameter | Bioprosthetic AVR (n = 97) Platelet levels | Prosthetic AVR (n = 100) Platelet levels | CABG (n = 100) Platelet levels | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| < 150 (n = 94) | ≥ 150 (n = 3) | P-value | < 150 (n = 72) | ≥ 150 (n = 28) | P-value | < 150 (n = 60) | ≥ 150 (n = 40) | P-value | ||||||||
| Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | Mean | SD | |||||
| Age [years] | 72.47 | 10.27 | 75.33 | 4.93 | - | 56.08 | 15.07 | 53.86 | 13.95 | d0.500 | 62.60 | 8.38 | 60.10 | 7.47 | d0.130 | |
| CPBT | 97.62 | 12.50 | 94.11 | 14.62 | d0.741 | 92.35 | 15.61 | 95.32 | 11.82 | d0.681 | 98.21 | 9.21 | 101.52 | 11.80 | d0.651 | |
| Hospitalization | 7.60 | 2.40 | 6.81 | 1.21 | d0.521 | 7.11 | 2.11 | 7.51 | 1.51 | d0.541 | 6.55 | 1.82 | 6.88 | 2.42 | d0.450 | |
| ICU stay | 1.2 | 1.1 | 1.3 | 1.5 | d0.841 | 1.1 | 1.51 | 1.38 | 1.11 | d0.451 | 1.17 | 1.02 | 1.32 | 1.41 | d0.661 | |
| Parameter | n | % | n | % | P-value | n | % | n | % | P-value | n | % | n | % | P-value | |
| Gender, n (%) | Female | 40 | 100 | 0 | 0 | e0.265 | 20 | 74.1 | 7 | 25.9 | f0.809 | 11 | 64.7 | 6 | 35.3 | c0.789 |
| Male | 54 | 94.7 | 3 | 5.3 | 52 | 71.2 | 21 | 28.8 | 49 | 59.0 | 34 | 41.0 | ||||
Among the risk factors thought to affect platelet levels falling below 150 (1000/mm3), age, gender and the type of surgery were found to be statistically significant when evaluated by enter logistic regression analysis (p < 0.01). In the model, the effect of bioprosthetic valve use was found significant (p < 0.01). The odds ratio of bioprosthetic valve use was found to be 16.779 (95% CI: 4.816–58.548), which means that bioprosthetic valve use has a 16.779 times more impact on causing platelet levels to fall below 150 (1000/mm3) when compared to CABG. The effect of mechanical prosthesis use was also very close to the level of significance and the odds ratio was found to be 1.842 (95% CI: 0.985–3.442). Age and gender effects were not significant (p > 0.05) (Table VIII).
Table VIII
Logistic regression analysis of risk factors affecting platelet levels < 150 (1000/mm3)
Discussion
Among the possible causes of thrombocytopenia following bioprosthetic AVR, the effect of CPB is undeniable. It is known that CPB affects both platelet count and function adversely. Studies have shown that the platelet count decreases rapidly to 50% of the preoperative levels shortly after CPB has been instituted [12].
A study by Hilker et al. reported that the time for postoperative thrombocyte counts to return to preoperative levels differed with regard to the type of implanted aortic valve prosthesis. This suggests that the material used during the construction of different prosthetic valves is responsible. Although these observations supported the association between platelet activation and bioprosthesis variants, the cause-and-effect relationship was not clearly established [13].
A study of the relationship between valve prosthesis types and thrombocytopenia by Ravenni et al. revealed a decrease in platelet counts in all bioprosthesis types on postoperative day 1. In comparison with Medtronic Mosaic (MOS) and Sorin Mitroflow (MIT) valves, the significant decrease in thrombocyte counts continued through the postoperative 3rd to 5th days in patients with a Sorin Freedom Solo (SFS) prosthesis [14].
In a double-center study conducted by Repossini et al. on 254 patients, it was reported that thrombocytopenia developed after implantation of the Sorin Freedom Solo valve prosthesis. It was determined that thrombocytopenia improved over 10 to 15 days without specific treatment, which was considered to be a transient phenomenon. It has been stated in a study that there is a relationship between the prosthetic valve size and the decrease in platelet levels. It has been reported that the probability of postoperative thrombocytopenia is reduced in patients using larger valve prostheses [15]. This may be due to turbulence which is typically seen in all types of prosthetic valves and is more severe in the small size ring [16]. In their study using Perceval sutureless aortic valves, Mujtaba et al. found a significant decrease in platelet count, requiring platelet suspension and erythrocyte transfusion [17].
However, in our study, no statistically significant relationship was found between the valve measurements and the minimum PLT and time to minimum (r = 0.009; r = –0.016; p > 0.05). Here was no statistically significant relation between the measurements of the valve and the preop-minimum PLT differences (r = 0.012; p > 0.05) (Table IX).
Table IX
Evaluation of relationship between cover measurements and PLT measurements in biological AVR group (n = 97)
| Parameter | Size | |
|---|---|---|
| Minimum PLT | 'r | 0.009 |
| p | 0.930 | |
| Time to reach the minimum [days] | r | –0.016 |
| p | 0.880 | |
| Difference preop-min. PLT | r | 0.012 |
| p | 0.908 | |
A study by Stanger et al. compared the thrombocytopenia observed after bioprosthetic valve operations in which stented, stentless, and sutureless bioprostheses were used. In this study, the use of Pericarbon Freedom, Perceval S and SOLO bioprosthetic valves showed a marked decrease in the postoperative platelet counts as compared to non-Sorin valves. However, the use of these valves has not been associated with bleeding complications, and they stated that this is a transient side effect on platelets [18]. In a study by Yerebakan et al. in which platelet counts after implantation of stentless (Sorin Freedom Solo) and stented (Sorin Mitroflow) bovine pericardial bioprostheses were compared, the mean platelet count in the Sorin Mitroflow group was moderately lower than the baseline values on the postoperative 3rd day whereas the platelet loss was more pronounced in the Sorin Freedom Solo group. They also observed that the thrombocytopenia was recovered without causing any major problem. A study of the relationship between antiaggregant drug therapy and platelet count by Yerebakan et al. reported that while clopidogrel was added to the treatment on the 3rd postoperative day, a serious decrease in platelet count had already been observed before clopidogrel treatment was started. In the same study, no correlation was observed between decreased platelet counts in patients receiving clopidogrel compared to other patients [19].
Another study, by Repossini et al., reported analysis of platelet activation with two different bioprostheses [Sorin Freedom Solo (FS) and Carpentier-Edwards Magna (CE)] after AVR. Postoperative changes in such parameters as platelet count, prothrombin, P-selectin, and b-TG were followed to test platelet activation and blood coagulation activation. They found that the platelet count was significantly reduced after Freedom Solo implantation compared to the other group. However, the factors involved in platelet activation and blood coagulation activation did not change significantly, indicating that the implanted bioprosthesis did not affect platelet and blood coagulation activation [20].
In a study that established the hypothesis that heparin-induced thrombocytopenia (HIT) might be responsible for thrombocytopenia seen after Freedom Solo bioprosthesis implantation, HIT antibody was investigated in half of the patients. No HIT antibody was detected in any patient. Forty percent of these patients had been treated with danaparoid. However, alteration of anticoagulation therapy was found to have no effect on the platelet count [19]. The same study also referred to glutaraldehyde, a storage solution for the bioprosthesis valve, and suggested that the toxic effect of glutaraldehyde on thrombocytes on glutaraldehyde-fixed bioprosthetic valves could not be removed by rinsing.
The results of our study strongly suggest that postoperative thrombocytopenia in the three groups of patients we examined is a transient phenomenon that spontaneously recovers within one week without any intervention and in which no recurrence is observed in late follow-up (including routine post-discharge visits). In our study, thrombocytopenia was observed in 96.9% of patients with bioprosthetic AVR. This rate was 72% in patients with mechanical prosthetic AVR (Table I). The incidence of thrombocytopenia in the bioprosthetic AVR group was significantly higher than in the mechanical prosthetic AVR (p = 0.001) and CABG (p = 0.001) groups (p < 0.01) (Table IV). Severe thrombocytopenia (0–50 (1000/mm3)) was observed in 21.6% of patients with bioprosthetic AVR. Patients who underwent mechanical prosthetic AVR and CABG did not have severe thrombocytopenia. In patients with bioprosthetic AVR, the lowest platelet levels were observed on the postoperative second day. In terms of differences between the preoperative and the lowest postoperative platelet measurements, the values of the bioprosthetic AVR group were significantly higher than the mechanical prosthetic AVR (p = 0.001) and CABG (p = 0.001) groups (p < 0.01) (Table IV). There was no statistically significant difference between platelet levels according to bioprosthetic valve sizes (p > 0.05) (Table VI). We investigated the relationship between the bleeding complications requiring postoperative intervention and the postoperative platelet count. There was no statistically significant relationship between the presence of a bleeding complication requiring postoperative intervention and the postoperative lowest platelet counts (p > 0.05). Causes of the number of patients undergoing reoperation due to bleeding being higher in the CABG group were thought to be specific to coronary artery bypass surgery, not related to thrombocytopenia. There was no statistically significant correlation between the presence of a bleeding complication requiring postoperative intervention and postoperative thrombocytopenia (p > 0.05) (Table III).
Other studies in this regard and our study show that thrombocytopenia is frequently observed after cardiac surgery employing CPB. In patients with bioprosthetic AVR, an increase in both the frequency and severity of postoperative thrombocytopenia was observed. This condition improved in about one week and the platelet count returned to preoperative values. The relationship between thrombocytopenia and a bleeding complication requiring postoperative intervention was not significant (p > 0.05).
The study we conducted was retrospective and single-centered, and the desired variety was not achieved in prosthetic valve types used in operations. An investigation of the coagulation system has not been done in these patients. Again, immunohistochemical and tissue analyses of the used prosthetic valves were not performed. A multicenter and detailed study of a larger patient population will provide more guidance in this regard.
Conclusions
As seen in our study, postoperative thrombocytopenia is a reality in patients undergoing bioprosthetic AVR. Thrombocytopenia after bioprosthetic AVR is considered to be a complex and multifactorial phenomenon, and more comprehensive and detailed studies are needed on the factors at play. In accordance with the literature, our study showed that thrombocytopenia following bioprosthetic AVR done with the same surgical techniques and principles recovers without causing any problem. It is postulated that the shear forces may cause washout of chemicals responsible for thrombocytopenia from the glutaraldehyde-treated bioprostheses.
Studies conducted up to this point suggest that suspicion of glutaraldehyde treatment during the construction of bioprosthetic valves is the cause of thrombocytopenia observed after bioprosthetic heart valve replacement. Although thrombocytopenia does not appear to have any effect on postoperative complications, caution should be exercised in the use of drugs affecting platelet number and function in these patients and precautions against bleeding problems should be taken.
