BADANIA KLINICZNE I DOŚWIADCZALNE W CHOROBACH SERCA, PŁUC I NACZYŃ

Background: Left ventricle surgical restoration is a promising therapy in patients with an ischaemically altered akinetic or dy-skinetic ventricle. Tissue engineered patches seeded with autologous bone morrow cells offer a potential tool for treatment of ischaemic cardiomyopathy in left ventricle surgical restoration therapy.

Aim: The aim of the study was to analyze the possibility to use in vitro differentiated bone morrow cells for the fabrication of tissue-engineered biological patches for use in left ventricle surgical restoration treatment.

Material and method: Bone morrow cells were isolated, cultured in different conditions and characterized in vitro. Tissue-engineered patches were fabricated by seeding these cells on an acellular scaffold. Uniaxial tensile test was used to estimate the biomechanical properties of the synthetic and biological patch. The test was performed in static and dynamic conditions.

Results: Bone marrow cells can be effectively isolated and differentiated in in vitro conditions and seeded on an acellular scaffold. These cells can be differentiated in the endothelial cell lineage. The uniaxial tensile test demonstrated a significant increase in the elastic modulus of the synthetic patch after the accelerated tensile test. No significant increase in the modulus was ob-served for the biological patch.

Conclusion: The results demonstrate that an acellular scaffold can be effectively tissue-engineered by bone morrow cells. The increase of elastic modulus in the synthetic patch after the accelerated tensile test can influence the haemodynamic properties of the left ventricle. This limits the use of the synthetic patch in left ventricle surgical restorations. The use of tissue-engineered patches can overcome the problems with synthetic patches.