BALB/C mice as a model for immunogenicity testing of food proteins
on the example of egg ovalbumin

Justyna Patrycja Chudzik-Kozłowska; Dagmara Złotkowska; Henryk Kostyra

doi:10.5114/ceji.2013.39757

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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4/2013
vol. 38

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abstract:

BALB/C mice as a model for immunogenicity testing of food proteins on the example of egg ovalbumin

Justyna Patrycja Chudzik-Kozłowska

,

Dagmara Złotkowska

,

Henryk Kostyra

(Centr Eur J Immunol 2013; 38 (4): 430-433)

DOI: https://doi.org/10.5114/ceji.2013.39757

Online publish date: 2013/12/30

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The aim of our study was to determine the dose and immunization scheme for commercial albumin from chicken egg white (OVA), based on immune response of mice gut mucosa. Balb/C mice were immunized orally in groups as follows: group I on day 0, 7, and 14 – 2 mg of OVA, group II on day 0, 7 and 14 – 100 µg of OVA, and group III on day 0, 1, 2, and 10 – 100 µg of OVA. After

37 days mice were intraperitoneally immunized with 100 µg OVA + CFA. We have demonstrated that blood serum titer for specific IgG was the highest in Group I and amounted to 213 and 211 after 14 and 35 days, respectively. Regardless of the immunization scheme, the dose of 100 µg induced lower response. All groups tested showed a slow decrease in IgG titer during four weeks of the experiment. By contrast, an intraperitoneal dose of 100 µg of OVA with CFA, administered on the 37th day of experiment, induced an increase in the end point titer (EPT) to 212-13 in all groups studied. What is more, stimulation of specific IgAs in blood serum and fecal extracts was on a detectable level. In conclusion, the selected method of antigen delivery was able to induce dose-dependent immune response. Therefore, the presented model of orally administered antigen without adjuvant can be used in the estimation of the immunogenicity of food proteins.

keywords:

BALB/C mice as a model for immunogenicity testing of food proteins on the example of egg ovalbumin

Justyna Patrycja Chudzik-Kozłowska , Dagmara Złotkowska , Henryk Kostyra

mucosal immunity, GALT, OVA, JgG, IgA

Justyna Patrycja Chudzik-Kozłowska

,

Dagmara Złotkowska

,

Henryk Kostyra