Bone marrow mesenchymal stem cell-originated exosomes suppress activation of hepatic stellate cells through the miR-144-3p/SLC7A11 axis

Yanqin Hao; Rong Wang; Qing Zhou; Jiaolong Ren

doi:10.5114/ceh.2024.142898

eISSN: 2449-8238
ISSN: 2392-1099

Clinical and Experimental Hepatology

Current issue Archive Manuscripts accepted About the journal Editorial board Subscription Contact Instructions for authors Ethical standards and procedures

Editorial System

Submit your Manuscript

Editorial Policies

Sarajevo Declaration on Integrity and Visibility of Scholarly Publications

3/2024
vol. 10

Send email

Copy url:

abstract:

Original paper

Bone marrow mesenchymal stem cell-originated exosomes suppress activation of hepatic stellate cells through the miR-144-3p/SLC7A11 axis

Yanqin Hao

¹

,

Rong Wang

²

,

Qing Zhou

²

,

Jiaolong Ren

¹

Department of Infectious Diseases, First Hospital of Shanxi Medical University, Taiyuan, Shanxi, P.R. China
The First Clinical Medical School, Shanxi Medical University, Taiyuan, Shanxi, P.R. China

Clin Exp HEPATOL 2024; 10, 3: 197-210

DOI: https://doi.org/10.5114/ceh.2024.142898

Online publish date: 2024/09/30

View full text Get citation

PlumX metrics:

Aim of the study:
This study aimed to investigate the impact of bone marrow-derived mesenchymal stem cell exosomes (BMSC-Exos) on hepatic stellate cell (HSC) activation and explore the underlying molecular mechanisms in liver fibrosis.

Material and methods:
BMSC-Exos were co-incubated with LPS-activated LX-2 cells. Fibrosis markers, iron content, malondialdehyde (MDA), glutathione (GSH), reactive oxygen species (ROS) levels, and ferroptosis-related proteins were assessed. The role of miR-144-3p originating from BMSC-Exos in LX-2 cell activation was studied through dual-luciferase reporter gene and RNA pull-down experiments.

Results:
Treatment with BMSC-Exos up-regulated miR-144-3p in LX-2 cells, down-regulated SLC7A11, increased iron content and ROS levels, and reduced fibrosis markers and GSH. BMSC-Exos mediated ferroptosis and inhibited HSC activation by transmitting miR-144-3p targeting SLC7A11.

Conclusions:
BMSC-Exos regulate SLC7A11 expression through miR-144-3p transfer, promoting ferroptosis and suppressing HSC activation in liver fibrosis.

keywords:

Bone marrow mesenchymal stem cell-originated exosomes suppress activation of hepatic stellate cells through the miR-144-3p/SLC7A11 axis

Yanqin Hao 1 , Rong Wang 2 , Qing Zhou 2 , Jiaolong Ren 1

bone marrow mesenchymal stem cells, exosomes, miR-144-3p, SLC7A11, ferroptosis, hepatic stellate cell activation

Yanqin Hao

¹

,

Rong Wang

²

,

Qing Zhou

²

,

Jiaolong Ren

¹