Cannabidiol modulation of immune cell function: in vitro insights and therapeutic implications for atopic dermatitis

Nada Chaoul; Stefano Palazzo; Alessandro Cinquantasei; Vincenzo Aresta; Concetta De Chirico; Marcello Albanesi

doi:10.5114/ada.2024.142182

eISSN: 2299-0046
ISSN: 1642-395X

Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii

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4/2024
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abstract:

Original paper

Cannabidiol modulation of immune cell function: in vitro insights and therapeutic implications for atopic dermatitis

Nada Chaoul

^{1, 2, 3}

,

Stefano Palazzo

^{1, 2, 4}

,

Alessandro Cinquantasei

^{1, 2}

,

Vincenzo Aresta

^{1, 2}

,

Concetta De Chirico

^{1, 2, 5}

,

Marcello Albanesi

^{1, 2, 6}

“M. Albanesi” Allergy and Immunology Unit, Bari, Italy
The Allergist, Bari, Italy
Department of Interdisciplinary Medicine, University of Bari, Bari, Italy
Faculty of Technological and Innovation Sciences, Universitas Mercatorum, Rome, Italy
University of Foggia, Italy
Allergolys, Asnieres-sur-Seine, France

Adv Dermatol Allergol 2024; XLI (4): 408-414

DOI: https://doi.org/10.5114/ada.2024.142182

Online publish date: 2024/08/12

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AMA

Chaoul N, Palazzo S, Cinquantasei A, Aresta V, De Chirico C, Albanesi M. Cannabidiol modulation of immune cell function: in vitro insights and therapeutic implications for atopic dermatitis. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2024;41(4):408-414. doi:10.5114/ada.2024.142182.

APA

Chaoul, N., Palazzo, S., Cinquantasei, A., Aresta, V., De Chirico, C.,  & Albanesi, M. (2024). Cannabidiol modulation of immune cell function: in vitro insights and therapeutic implications for atopic dermatitis. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 41(4), 408-414. https://doi.org/10.5114/ada.2024.142182

Chicago

Chaoul, Nada, Stefano Palazzo, Alessandro Cinquantasei, Vincenzo Aresta, Concetta De Chirico,  and Marcello Albanesi. 2024. "Cannabidiol modulation of immune cell function: in vitro insights and therapeutic implications for atopic dermatitis". Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii 41 (4): 408-414. doi:10.5114/ada.2024.142182.

Harvard

Chaoul, N., Palazzo, S., Cinquantasei, A., Aresta, V., De Chirico, C.,  and Albanesi, M. (2024). Cannabidiol modulation of immune cell function: in vitro insights and therapeutic implications for atopic dermatitis. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, 41(4), pp.408-414. https://doi.org/10.5114/ada.2024.142182

MLA

Chaoul, Nada et al. "Cannabidiol modulation of immune cell function: in vitro insights and therapeutic implications for atopic dermatitis." Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii, vol. 41, no. 4, 2024, pp. 408-414. doi:10.5114/ada.2024.142182.

Vancouver

Chaoul N, Palazzo S, Cinquantasei A, Aresta V, De Chirico C, Albanesi M. Cannabidiol modulation of immune cell function: in vitro insights and therapeutic implications for atopic dermatitis. Advances in Dermatology and Allergology/Postępy Dermatologii i Alergologii. 2024;41(4):408-414. doi:10.5114/ada.2024.142182.

Introduction:
Cannabidiol (CBD) exhibits neuroprotective, anti-inflammatory, and immunomodulatory properties, making it a promising candidate for addressing inflammatory skin disorders like atopic dermatitis.

Aim:
This study aimed to (i) investigate CBD’s impact on lymphocyte proliferation and lymphocyte viability; ii) assess in vitro cytotoxicity U937 cells? (a human promonocytic cell line) of CBD/cytotoxicity of CBD on U937 cells; (iii) provide insights into CBD immunomodulatory potential, and (iv) evaluate suitability of CBD for treating inflammatory skin conditions.

Material and methods:
To this aim PBMCs from healthy donors were cultured with mitogen and two different CBD doses (0.1 and 1 mg/ml), assessing B and T cell proliferation through flow cytometry. CBD inhibited mitogen-induced lymphocyte proliferation, reducing the percentage of proliferating T and B cells. Notably, both CBD doses did not exhibit cytotoxicity on lymphocytes as revealed by viability assessment. We also analysed the effect of CBD on U937 cells using an optical microscopy approach. Interestingly, the higher dose of CBD exerted a cytotoxic effect on U937 cells, while the lower dose was well tolerated.

Results:
Finally, we analysed the effect of an adjuvant treatment for atopic dermatitis with a CBD-containing cleansing cream in reducing itch. Notably, the treatment with the CBD-containing cleansing cream significantly reduced itch in patients suffering from atopic dermatitis.

Conclusions:
These findings affirm CBD’s immunomodulatory characteristics, emphasizing its potential therapeutic application in inflammatory skin disorders.

keywords:

Cannabidiol modulation of immune cell function: in vitro insights and therapeutic implications for atopic dermatitis

Nada Chaoul 1, 2, 3 , Stefano Palazzo 1, 2, 4 , Alessandro Cinquantasei 1, 2 , Vincenzo Aresta 1, 2 , Concetta De Chirico 1, 2, 5 , Marcello Albanesi 1, 2, 6

cannabidiol, cannabinoid, lymphocyte, proliferation, cytotoxicity, atopic dermatitis

Nada Chaoul

^{1, 2, 3}

,

Stefano Palazzo

^{1, 2, 4}

,

Alessandro Cinquantasei

^{1, 2}

,

Vincenzo Aresta

^{1, 2}

,

Concetta De Chirico

^{1, 2, 5}

,

Marcello Albanesi

^{1, 2, 6}