eISSN: 1644-4124
ISSN: 1426-3912
Central European Journal of Immunology
Current issue Archive Manuscripts accepted About the journal Special Issues Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
Search
1/2015
vol. 40
 
Share:
Send email
Share:
abstract:

Clinical immunology
Prohibitin as a novel autoantigen in rheumatoid arthritis

Li-Li Shi
,
Peng Chen
,
Yi-Ping Xun
,
Wei-Kang Yang
,
Chun-He Yang
,
Guang-Yu Chen
,
Hong-Wu Du

(Centr Eur J Immunol 2015; 40 (1): 78-82)
DOI: https://doi.org/10.5114/ceji.2015.50837
Online publish date: 2015/04/22
View full text Get citation
 
PlumX metrics:
The aim of this study was to verify whether prohibitin is a novel autoantigen in rheumatoid arthritis.

Material and methods: First, recombinant human prohibitin (rhPHB) protein was cloned, expressed, and purified. Then the anti-prohibitin autoantibodies were detected by western blotting by using rhPHB protein to incubate sera from patients with rheumatoid arthritis (RA). Next, immunoprecipitation was employed to further illustrate whether anti-prohibitin antibodies exist in RA patients. And finally, autoantibodies against the rhPHB protein were investigated using a homemade ELISA kit through the assessment of 258 real clinical samples.

Results: It was revealed that anti-prohibitin antibodies existed in the sera of patients with RA. Reactivity of serum IgG against rhPHB was detected in 26 of 86 RA patients (30.3%), 7 of 86 systemic lupus erythematosus (SLE) patients (8.1%), and 1 of 86 apparently healthy donors (HC) (1.2%).

Conclusions: Prohibitin was proved to be a novel autoantigen and the corresponding anti-prohibitin autoantibodies were present in the RA patients’ blood circulation.
keywords:

autoantibodies, autoantigen, autoimmune disease, prohibitin, rheumatoid arthritis
Termedia
About us Contact
Copyright notice Privacy policy Advertising policy Contact us
Quick links
Journals Books eBooks Events
© 2024 Termedia Sp. z o.o.
Developed by Bentus.