Comparison of T-cell receptor β chain variable region 23-1 open reading frame and 3-1 F CDR3 repertoire in cDNA and gDNA from peripheral blood mononuclear cells of healthy volunteers by high-throughput sequencing

He X, Shi B, Ma L, et al. Comparison of T-cell receptor β chain variable region 23-1 open reading frame and 3-1 F CDR3 repertoire in cDNA and gDNA from peripheral blood mononuclear cells of healthy volunteers by high-throughput sequencing. Central European Journal of Immunology. 2018;43(3):295-305. doi:10.5114/ceji.2018.80049.

APA

He, X., Shi, B., Ma, L., Ma, R., Peng, A.,  & Sun, S. et al. (2018). Comparison of T-cell receptor β chain variable region 23-1 open reading frame and 3-1 F CDR3 repertoire in cDNA and gDNA from peripheral blood mononuclear cells of healthy volunteers by high-throughput sequencing. Central European Journal of Immunology, 43(3), 295-305. https://doi.org/10.5114/ceji.2018.80049

Chicago

He, Xiaoyan, Bin Shi, Long Ma, Rui Ma, Aihua Peng, Suhong Sun,  and Xinsheng Yao. 2018. "Comparison of T-cell receptor β chain variable region 23-1 open reading frame and 3-1 F CDR3 repertoire in cDNA and gDNA from peripheral blood mononuclear cells of healthy volunteers by high-throughput sequencing". Central European Journal of Immunology 43 (3): 295-305. doi:10.5114/ceji.2018.80049.

Harvard

He, X., Shi, B., Ma, L., Ma, R., Peng, A., Sun, S.,  and Yao, X. (2018). Comparison of T-cell receptor β chain variable region 23-1 open reading frame and 3-1 F CDR3 repertoire in cDNA and gDNA from peripheral blood mononuclear cells of healthy volunteers by high-throughput sequencing. Central European Journal of Immunology, 43(3), pp.295-305. https://doi.org/10.5114/ceji.2018.80049

MLA

He, Xiaoyan et al. "Comparison of T-cell receptor β chain variable region 23-1 open reading frame and 3-1 F CDR3 repertoire in cDNA and gDNA from peripheral blood mononuclear cells of healthy volunteers by high-throughput sequencing." Central European Journal of Immunology, vol. 43, no. 3, 2018, pp. 295-305. doi:10.5114/ceji.2018.80049.

Vancouver

He X, Shi B, Ma L, Ma R, Peng A, Sun S et al. Comparison of T-cell receptor β chain variable region 23-1 open reading frame and 3-1 F CDR3 repertoire in cDNA and gDNA from peripheral blood mononuclear cells of healthy volunteers by high-throughput sequencing. Central European Journal of Immunology. 2018;43(3):295-305. doi:10.5114/ceji.2018.80049.

PlumX metrics:

The TRBV germline gene can be classified into three types: open reading frame (ORF), functional gene (F), and pseudogene (P); however, the differences and connections among ORF, functional gene, and pseudogene rearrangements and expression characteristics remain unclear. Here, we compared the characteristics of the CDR3 repertoire that was rearranged by TRBV23-1(ORF) and TRBV3-1(F) in four cDNA samples and six gDNA samples from healthy volunteers. In this study, we show that the frequencies of in-frame sequences in the TRBV23-1 complementarity determining region 3 (CDR3) repertoire were significantly lower than those in the TRBV3-1 CDR3 repertoire. The TRBV23-1 CDR3 repertoire, which differed from the TRBV3-1 in-frame and out-of-frame CDR3 repertoire, consisted of 1/3 in-frame sequences and 2/3 out-of-frame sequences. The usage of TRBD1 was higher than that of TRBD2 in the TRBV23-1 in-frame and out-of-frame CDR3 repertoire. In four cDNA samples from PBMCs, the TRBV23-1 in-frame and out-of-frame CDR3 repertoire showed a longer N2 relative to that of N1. Therefore, our results demonstrate that the mechanisms of rearrangement in the TRBV23-1 and TRBV3-1 CDR3 repertoire were different, and thus offered new ideas and methods to resolve the discrepancies in reports on the functionality of TRBV23-1 and to better understand the mechanisms underlying VDJ recombination.

keywords:

Comparison of T-cell receptor β chain variable region 23-1 open reading frame and 3-1 F CDR3 repertoire in cDNA and gDNA from peripheral blood mononuclear cells of healthy volunteers by high-throughput sequencing

Xiaoyan He , Bin Shi , Long Ma , Rui Ma , Aihua Peng , Suhong Sun , Xinsheng Yao

T-cell receptor, T-cell receptor β variable, complementarity-determining region 3 repertoire, open reading frame, high-throughput sequencing

Xiaoyan He

,

Bin Shi

,

Long Ma

,

Rui Ma

,

Aihua Peng

,

Suhong Sun

,

Xinsheng Yao