eISSN: 2353-9461
ISSN: 0860-7796
BioTechnologia
Current issue Archive About the journal Editorial board Abstracting and indexing Subscription Contact Instructions for authors Publication charge Ethical standards and procedures
Editorial System
Submit your Manuscript
SCImago Journal & Country Rank
1/2022
vol. 103
 
Share:
Share:
abstract:
RESEARCH PAPERS

Effect of juglone on C-32 and COLO 829 melanoma cells in in vitro cultures

Aleksandra Zielińska
1
,
Justyna Płonka-Czerw
2
,
Dariusz Kuśmierz
2

  1. Department of Medical Genetics, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Sosnowiec, Poland
  2. Department of Cell Biology, Faculty of Pharmaceutical Sciences in Sosnowiec, Medical University of Silesia, Sosnowiec, Poland
BioTechnologia vol. 103(1) ∙ pp. 29–39 ∙ 2022
Online publish date: 2022/03/24
View full text Get citation
 
PlumX metrics:
Juglone is an allelopathin secreted by black walnut tree of the Juglandaceae family and is used as an active ingredient in many herbal preparations and as a commercial dye. It is considered as an important phytochemical with wide therapeutic potential. Black walnut extract has long been used in folk medicine to treat various types of cancers. It demonstrates antiviral, antibacterial, antifungal, and antitumor activities. The present study aimed to analyze the effect of juglone on the viability and proliferation of melanoma cells of C-32 (amelanotic melanoma) and COLO 829 (melanotic melanoma) cell lines in vitro and on the mRNA expression of genes encoding the proapoptotic BAX protein and caspase 3 and the gene encoding antiapoptotic BCL2 protein. The results showed a dose-dependent effect of juglone on the viability, proliferation, and death induction in C-32 and COLO 829 melanoma cells and in HFF-1 normal dermal fibroblasts in in vitro cultures, but melanoma cells were more sensitive to juglone. Our findings revealed different mRNA expression patterns for all the studied genes in melanoma and normal cells treated with juglone in in vitro cultures.
keywords:

juglone, cell cultures, melanoma, BAX gene, BCL2 gene, CASP3 gene



Stosujemy się do standardu HONcode dla wiarygodnej informacji zdrowotnej This site complies with the HONcode standard for trustworthy health information: verify here