Effects of miR-211-3p/RHBDD1 axis on cell proliferation, 
cell cycle progression, and epithelial-mesenchymal transition 
in glioma

Lei Chen; Xiangyi Wang; Yude Zhu; Fuquan Liu; Laixing Liu; Ning Jiang

doi:10.5114/fn.2022.118186

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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2/2022
vol. 60

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abstract:

Original paper

Effects of miR-211-3p/RHBDD1 axis on cell proliferation, cell cycle progression, and epithelial-mesenchymal transition in glioma

Lei Chen

¹

,

Xiangyi Wang

¹

,

Yude Zhu

¹

,

Fuquan Liu

¹

,

Laixing Liu

¹

,

Ning Jiang

¹

Department of Neurosurgery, Inner Mongolia Baogang Hospital, Baotou City, Inner Mongolia Autonomous Region, P.R. China

Folia Neuropathol 2022; 60 (2): 195-209

DOI: https://doi.org/10.5114/fn.2022.118186

Online publish date: 2022/06/30

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This study was designed to elucidate the relationship of miR-211-3p and rhomboid domain containing 1 (RHBDD1) in glioma. Here, we first observed that miR-211-3p directly targets the 3˘-UTR of RHBDD1 in glioma cells using dual-luciferase reporter assay, RNA immunoprecipitation (RIP) assay, reverse transcription-quantitative polymerase chain reaction (RT-qPCR), and Western blot analysis. Pearson’s correlation analysis showed that miR-211-3p expression was negatively correlated with RHBDD1 expression in glioma tissues. CCK-8 assay, flow cytometry, and transwell assay were applied to assess cell proliferation, cell cycle distribution, migration, and invasion. The results showed that RHBDD1 knockdown inhibited cell proliferation, cell cycle G1/S transition, migration, and invasion in two glioma cell lines (U87 and LN-229). Knockdown of miR-211-3p obtained opposite results. Moreover, overexpression of RHBDD1 counteracted suppressive effects of miR-211-3p on glioma cells. Furthermore, decreased expression of CDK4, cyclin D1, N-cadherin, and vimentin as well as increased E-cadherin expression induced by miR-211-3p was reversed by RHBDD1 overexpression. Our results suggested that targeting miR-211-3p/RHBDD1 axis might be a novel effective therapeutic target for glioma treatment.

keywords:

Effects of miR-211-3p/RHBDD1 axis on cell proliferation, cell cycle progression, and epithelial-mesenchymal transition in glioma

Lei Chen 1 , Xiangyi Wang 1 , Yude Zhu 1 , Fuquan Liu 1 , Laixing Liu 1 , Ning Jiang 1

glioma, miR-211-3p, RHBDD1, proliferation, EMT

Lei Chen

¹

,

Xiangyi Wang

¹

,

Yude Zhu

¹

,

Fuquan Liu

¹

,

Laixing Liu

¹

,

Ning Jiang

¹