Experimental immunology<br>The activity of phagocytic cells after <i>in vivo</i> administration of propentofylline in mice

Marianna Szczypka; Magdalena Lis; Agnieszka Suszko; Aleksandra Pawlak; Bożena Obmińska-Mrukowicz

doi:10.5114/ceji.2013.35210

eISSN: 1644-4124
ISSN: 1426-3912

Central European Journal of Immunology

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2/2013
vol. 38

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abstract:

Experimental immunology
The activity of phagocytic cells after in vivo administration of propentofylline in mice

Marianna Szczypka

,

Magdalena Lis

,

Agnieszka Suszko

,

Aleksandra Pawlak

,

Bożena Obmińska-Mrukowicz

(Centr Eur J Immunol 2013; 38 (2): 169-174)

DOI: https://doi.org/10.5114/ceji.2013.35210

Online publish date: 2013/07/08

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Propentofylline, a phosphodiesterase (PDE) inhibitor, increases the intracellular level of cyclic nucleotides (cAMP and cGMP) and, in consequence, can change the activity of many cells, including immune cells. The studies were conducted on female Balb/c mice (8 weeks of age). Propentofylline was administered orally once or six times at 12 h intervals at a therapeutic dose of 3 mg/kg. The production of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β) and nitric oxide (NO) by resident peritoneal murine macrophages stimulated in vitro with lipopolisaccharide (LPS) from Escherichia coli and the phagocytic activity of granulocytes and monocytes from peripheral blood were determined 12 h and 24 h after a single dose or after the last of six doses of propentofylline administration. A temporary decrease in TNF-α synthesis and an increase in IL-1β production were noted 12 h following a single administration of propentofylline. No effect on the TNF-α and IL-1β release was observed after six doses of the drug applied. Propentofylline, irrespective of the number of subsequent doses applied, did not change the synthesis and release of NO by peritoneal murine macrophages stimulated in vitro with LPS. No effect on the percentage of phagocytosing granulocytes and monocytes was observed after a single administration of propentofylline. However, an increase in the fluorescence intensity of the granulocytes was observed 12 h and 24 h after a single dose of the drug administered. Propentofylline administered six times did not change the fluorescence intensity of granulocytes and monocytes, but an increase in the percentage of phagocytosing monocytes was noted. The results obtained in the present study showed that propentofylline administered in vivo did not exhibit an immunosuppressive influence on the activity of phagocytic cells. The changes after propentofylline administration were short-lived and were balanced during the treatment.

keywords:

Experimental immunologyThe activity of phagocytic cells after in vivo administration of propentofylline in mice

Marianna Szczypka , Magdalena Lis , Agnieszka Suszko , Aleksandra Pawlak , Bożena Obmińska-Mrukowicz

propentofylline, phagocytosis, TNF-α, IL-1β, nitric oxide

Experimental immunology
The activity of phagocytic cells after in vivo administration of propentofylline in mice

Marianna Szczypka

,

Magdalena Lis

,

Agnieszka Suszko

,

Aleksandra Pawlak

,

Bożena Obmińska-Mrukowicz