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Folia Neuropathologica
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3/2021
vol. 59
 
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abstract:
Original paper

Expression of interferon regulatory factor 4 and inflammation in secondary injury of intracerebral haemorrhage

Bingqian Wang
1
,
Xiaopeng Li
2
,
Tianyu Dong
3
,
Feng Gao
1
,
Zhenzhong Li
1
,
Zenglu Ma
1

  1. Department of Neurosurgery, Affiliated Xing Tai People’s Hospital of Hebei Medical University, Xingtai, Hebei, China
  2. Department of Neurosurgery, The First Hospital of Handan City, Handan, Hebei, China
  3. Human Anatomy Department, Hebei Medical University, Shijiazhuang, Hebei, China
Folia Neuropathol 2021; 59 (3): 291-297
DOI: https://doi.org/10.5114/fn.2021.109200
Online publish date: 2021/10/05
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The aim of this study was to investigate the expression of interferon regulatory factor 4 (IRF4) and the inflammatory response in secondary injury of intracerebral haemorrhage (ICH). Twelve SD rats were randomly divided into a sham group and an ICH group, with 6 rats in each group. A rat model of ICH was established by injecting collagenase type IV into the right striatum of rats. The expression of IRF4 was measured by western blot and immunohistochemistry 48 h after ICH. In addition, 15 mm of hemin-induced PC12 cell injury was used to simulate an in vitro ICH model. IRF4 expression was detected by immunofluorescence (IF). Moreover, the inflammatory cytokines (IL-1b and IL-6) were measured by ELISA. The behavioural score of ICH rats was the lowest at 48 h after operation. The expression of IRF4 was significantly higher in the striatal tissue of ICH rats compared with the sham group (p < 0.05). Meanwhile, IF results showed that hemin induced the upregulation of IRF4 expression in rat pheochromocytoma cells PC12. In addition, IL-1b and IL-6 levels were significantly increased in the serum of ICH rats and in the supernatant of hemin-induced PC12 cells (p < 0.01). The inflammation in ICH is related to the increase of IRF4. It provides a theoretical basis for the clinical treatment of ICH.
keywords:

intracerebral haemorrhage (ICH), secondary injury, interferon regulatory factor 4 (IRF4), inflammation, treatment
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