Hydroxysafflor yellow A promotes α-synuclein clearance via regulating autophagy in rotenone-induced Parkinson’s disease mice

Bing Han; Lijie Wang; Fenghua Fu; Zhenhua Wang; Leiming Zhang; Grace Jia Qi; Tian Wang

doi:10.5114/fn.2018.76618

eISSN: 1509-572x
ISSN: 1641-4640

Folia Neuropathologica

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2/2018
vol. 56

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abstract:

Original paper

Hydroxysafflor yellow A promotes α-synuclein clearance via regulating autophagy in rotenone-induced Parkinson’s disease mice

Bing Han

,

Lijie Wang

,

Fenghua Fu

,

Zhenhua Wang

,

Leiming Zhang

,

Grace Jia Qi

,

Tian Wang

Folia Neuropathol 2018; 56 (2): 133-140

DOI: https://doi.org/10.5114/fn.2018.76618

Online publish date: 2018/06/28

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Autophagy is an intracellular degradation process that is involved in α-synuclein (α-syn) homeostasis and Parkinson’s disease (PD). The purpose of this study was to investigate whether hydroxysafflor yellow A (HSYA) could promote α-syn clearance via regulating autophagy in PD mice. Male C57BL/6 mice were intraperitoneally treated with HSYA. Thirty minutes later, they were intragastrically administered with rotenone at a dose of 30 mg/kg. The hanging wire test was performed at 14 and 28 days. Then, autophagosomes and ultrastructural changes were examined by transmission electron microscopy. The expression of tyrosine hydroxylase (TH), α-syn, JNK1, p-JNK1, Bcl-2, p-Bcl-2, Beclin1, autophagy-related proteins (Atg) 7 and 12-5, and the LC3-II/LC3-I ratio were investigated by western blot. The hanging time of HSYA-treated PD mice was significantly increased compared with that of rotenone-induced PD mice (p < 0.05 or p < 0.01). Compared with rotenone-induced PD mice, treatment with HSYA augmented the formation of autophagosomes. The expression of TH, p-JNK1/JNK1, Beclin1, Atg7, Atg12-5, p-Bcl-2/Bcl-2, and the LC3-II/LC3-I ratio were significantly increased, whereas the expression of α-syn was reduced in the rotenone plus HSYA group. These results indicate that HSYA promoted α-syn clearance via regulating autophagy in rotenone-induced PD mice.

keywords:

Hydroxysafflor yellow A promotes α-synuclein clearance via regulating autophagy in rotenone-induced Parkinson’s disease mice

Bing Han , Lijie Wang , Fenghua Fu , Zhenhua Wang , Leiming Zhang , Grace Jia Qi , Tian Wang

hydroxysafflor yellow A, rotenone, neurotoxicity, α-synuclein, autophagy

Bing Han

,

Lijie Wang

,

Fenghua Fu

,

Zhenhua Wang

,

Leiming Zhang

,

Grace Jia Qi

,

Tian Wang