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BioTechnologia
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1/2022
vol. 103
 
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abstract:
RESEARCH PAPERS

Impact of culture vessels on micro-morphological features of in vitro Dendrobium Sabin Blue orchid

Aimie Nadzirah Abd Malik
1
,
Jasim Uddain
2
,
Chee Keong Chin
1
,
Bee Lynn Chew
1
,
Jessica Jeyanthi James Antony
1
,
Ankita Rajendra Parab
1
,
Sreeramanan Subramaniam
1, 3, 4

  1. Centre for Chemical Biology, Universiti Sains Malaysia (USM), Bayan Lepas, Penang, Malaysia
  2. Institute of Nano Optoelectronics Research and Technology, Universiti Sains Malaysia (USM), Bayan Lepas, Penang, Malaysia
  3. School of Chemical Engineering Technology, Universiti Malaysia Perlis (UNIMAP), Arau, Perlis, Malaysia
  4. National Poison Centre, Universiti Sains Malaysia (USM), Peneng, Malaysia
BioTechnologia vol. 103(1) ∙ pp. 41–52 ∙ 2022
DOI: https://doi.org/10.5114/bta.2022.113914
Online publish date: 2022/03/24
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Different designs of the plant tissue culture vessel, such as size, material, and shape, may alter its microenvironment atmosphere. The present study was conducted on protocorm-like bodies (PLBs) of Dendrobium Sabin Blue orchid to determine the development of PLBs on plastic and glass culture vessels of different sizes. PLBs were cultured in half-strength Murashige and Skoog (MS) medium with the same initial weight of 0.5 g in 10 replicates. The growth index of the PLBs was calculated after 11 weeks to study their growth in every vessel; additionally, biochemical analysis was performed to determine carbohydrate content, proline concentration, and photosynthesis pigments in the PLBs. Scanning electron microscopy (SEM) was performed to study stomata development on PLBs in each vessel, and histological analyses were conducted to study the cell structure. Overall, the PLBs cultured in a large 470 ml plastic vessel showed successful growth with a high growth index, high carbohydrate content, low-stress condition, and high chlorophyll content. SEM confirmed that the presence of trichome and rhizoid in PLBs cultured in the 470 ml plastic vessel. Histological analysis showed the formation of the shoot on the PLBs and the presence of starch granules. Thus, the use of plastic as a culture vessel provides a good impact for culturing PLBs and has low cost.
keywords:

alternative culture vessel, orchid, in vitro culture, protocorm-like bodies (PLBs)


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